Modulation of Cholesterol-Related Gene Expression by Dietary Fiber Fractions from Edible Mushrooms
Modulation of Cholesterol-Related Gene Expression by Dietary Fiber Fractions from Edible Mushrooms
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DOI：
10.1021/acs.jafc.5b02942
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发表时间：
2015-08-26
影响因子：
6.1
通讯作者：
Soler-Rivas, Cristina
中科院分区：
文献类型：
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作者：
Caz, Victor;Gil-Ramirez, Alicia;Soler-Rivas, Cristina
蘑菇是膳食纤维（DF）的来源，具有降低胆固醇的作用。然而，人们对它们的潜在机制知之甚少。在体外研究了三种蘑菇中富含df的组分对胆固醇相关表达的影响。用平菇DF （PDF）在小鼠模型中评估其对高胆固醇血症的潜在缓解或预防作用。PDF在Caco-2细胞中诱导转录反应，提示可能具有降低胆固醇的作用。在姑息条件下，PDF降低肝脏甘油三酯可能是因为Dgat1下调。然而，胆固醇相关的生化数据显示没有变化，也与观察到的转录调节无关。在预防设置中，PDF以类似于辛伐他汀和依泽替米贝的方式调节胆固醇相关基因在肝脏中的表达，尽管没有引起血浆和肝脏生化数据的变化。因此，PDF可能有助于减少肝脏甘油三酯的积累。由于它在肝脏中诱导的分子反应类似于降胆固醇药物，因此应进行进一步的剂量依赖性研究。
Mushrooms are a source of dietary fiber (DF) with a cholesterol-lowering effect. However, their underlying mechanisms are poorly understood. The effect of DF-enriched fractions from three mushrooms species on cholesterol-related expression was studied in vitro. The Pleurotus ostreatus DF fraction (PDF) was used in mice models to assess its potential palliative or preventive effect against hypercholesterolemia. PDF induced a transcriptional response in Caco-2 cells, suggesting a possible cholesterol-lowering effect. In the palliative setting, PDF reduced hepatic triglyceride likely because Dgat1 was down-regulated. However, cholesterol-related biochemical data showed no changes and no relation with the observed transcriptional modulation. In the preventive setting, PDF modulated cholesterol-related genes expression in a manner similar to that of simvastatin and ezetimibe in the liver, although no changes in plasma and liver biochemical data were induced. Therefore, PDF may be useful reducing hepatic triglyceride accumulation. Because it induced a molecular response similar to hypocholesterolemic drugs in liver, further dose-dependent studies should be carried out.