The epidermal nerve fibre network: characterization of nerve fibres in human skin by confocal microscopy and assessment of racial variations
The epidermal nerve fibre network: characterization of nerve fibres in human skin by confocal microscopy and assessment of racial variations
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DOI：
10.1046/j.1365-2133.1997.18001893.x
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发表时间：
1997-08-01
影响因子：
10.3
通讯作者：
Green, MR
中科院分区：
文献类型：
--
作者：
Reilly, DM;Ferdinando, D;Green, MR
作为第一道防线，皮肤配备了复杂和相互作用的神经纤维系统，以检测刺激物并保持体内平衡。这种纤维网络的真皮成分已被很好地表征，并且已知纤维作为游离神经末梢在整个有活力的表皮中延伸。迄今为止，这种表皮成分的特征仍然很差。我们通过激光扫描共聚焦显微镜，使用泛神经元标记物、蛋白基因产物9.5和P物质、降钙素基因相关肽和神经生长因子的特异性抗体，观察了人前臂掌侧表皮神经纤维。除了静脉曲张的游离神经末梢外，正常人表皮中存在三维纤维网络，纤维频繁分支，可以看到分支聚集在明显延伸到真皮的中央主干上，在活表皮的所有层中都可以看到薄的无髓鞘纤维，很少观察到P物质染色并且比蛋白质基因产物9.5染色弱得多，降钙素基因相关肽和神经生长因子在前臂掌侧表皮中未检测到。用神经药理学试剂预处理体内皮肤。辣椒素，导致表皮纤维染色的损失，表明这些是初级C-传入类型的感觉纤维。还评估了种族和人种皮肤类型中的表皮神经支配。在欧洲高加索人和日本人/中国人皮肤之间在结构或生化水平上没有观察到神经支配的明显差异，即在所有测试的情况下，纤维的存在、性质和生化含量是相似的。
As a first line of defence, the skin is equipped with a complex and interactive nerve fibre system to detect irritants and maintain homeostasis. The dermal component of this fibre network has been well characterized and fibres are known to extend throughout the viable epidermis as free nerve endings. To date, this epidermal component remains poorly characterized, We have visualized human volar forearm epidermal nerve fibres by laser-scanning confocal microscopy using the pan-neuronal marker, protein gene-product 9.5 and specific antibodies to substance P, calcitonin gene-related peptide and nerve growth factor, In addition to the varicose free nerve endings, there is a 3-D fibre network in normal human epidermis, with frequent branching of fibres, Branching can be seen to converge on a central trunk apparently extending to the dermis, Thin unmyelinated fibres can be seen in all layers of the viable epidermis, Substance P staining is rarely observed and is much less intense than the protein gene-product 9.5 staining, Calcitonin gene-related peptide and nerve growth factor were not detected in volar forearm epidermis by this method.Pretreatment of the skin in vivo with the neuropharmacological agent. capsaicin, resulted in loss of epidermal fibre staining indicating that these are sensory fibres of the primary C-afferent type, Epidermal innervation in racial and ethnic skin types was also assessed, No apparent difference in innervation was observed between European caucasian and Japanese/Chinese skin at the architectural or biochemical level, i.e. the presence, properties and biochemical content of fibres was similar in all cases tested.