Integrated transcriptome analysis reveals miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma.
Integrated transcriptome analysis reveals miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma.
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DOI：
10.1016/j.ygeno.2014.06.004
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发表时间：
2014-10
期刊：
影响因子：
4.4
通讯作者：
Yang Zhang;Yong Chen;Jin-hai Yu;Gui Liu;Zhigang Huang
中科院分区：
文献类型：
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作者：
Yang Zhang;Yong Chen;Jin-hai Yu;Gui Liu;Zhigang Huang
下一代测序（NGS）已被证明是一个强大的工具，在描绘无数的分子亚型的癌症，以及在揭示积累的基因组突变在整个癌症的进展。采用深度测序技术获得喉鳞状细胞癌（LSCC）患者全基因组microRNA（miRNA）和mRNA表达谱，并利用整合计算方法进行分析。大量的蛋白质编码基因和非编码基因被检测到差异表达，表明在喉鳞状细胞癌细胞的功能开关。共检测到127个突变基因与外胚层和表皮发育显著相关。发现11种miRNA差异表达，包括潜在的癌症抑制miRNA mir-34 c，其显著下调。mRNA和miRNA转录组的综合分析进一步揭示了11个miRNA和138个靶基因之间的相关动力学，形成了一个高度动态的协同调控网络，以响应LSCC的发展。
Next generation sequencing (NGS) has proven to be a powerful tool in delineating myriads of molecular subtypes of cancer, as well as in revealing accumulation of genomic mutations throughout cancer progression. Whole genome microRNA (miRNA) and mRNA expression profiles were obtained from patients with laryngeal squamous cell carcinoma (LSCC) using deep sequencing technology, and were analyzed by utilizing integrative computational approaches. A large number of protein-coding and non-coding genes were detected to be differentially expressed, indicating a functional switch in LSCC cells. A total of 127 mutated genes were detected to be significantly associated with ectoderm and epidermis development. Eleven miRNAs were found to be differentially expressed, including a potential cancer suppressor miRNA, mir-34c, which was dramatically down-regulated. Integrated analysis of mRNA and miRNA transcriptomes further revealed correlated dynamics among 11 miRNAs and 138 targeted genes, forming a highly dynamical co-regulation network response to LSCC development.