Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations.
Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations.
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DOI：
10.1177/2041731416650838
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发表时间：
2016-01
影响因子：
8.2
通讯作者：
Vargis E
中科院分区：
文献类型：
--
作者：
Fronk AH;Vargis E
视网膜色素上皮是脊椎动物眼睛的重要组成部分，特别是在研究年龄相关性黄斑变性的原因和可能的治疗方法方面。视网膜色素上皮由于其位于眼睛后部而难以在体内进入，使得年龄相关性黄斑变性治疗的实验存在问题。体内实验的替代方案是体外培养视网膜色素上皮，这是自20世纪70年代以来一直在进行的实践，提供了广泛的视网膜色素上皮培养方案，每个方案产生与天然视网膜色素上皮不同程度相似的细胞和组织。这篇综述的目的是为研究人员提供一个现成的视网膜色素上皮协议，他们对培养组织的影响，以及他们的具体可能的应用。协议使用人类和动物视网膜色素上皮细胞，来自组织或细胞系，进行了讨论，并建议为未来的研究人员。
The retinal pigment epithelium is an important part of the vertebrate eye, particularly in studying the causes and possible treatment of age-related macular degeneration. The retinal pigment epithelium is difficult to access in vivo due to its location at the back of the eye, making experimentation with age-related macular degeneration treatments problematic. An alternative to in vivo experimentation is cultivating the retinal pigment epithelium in vitro, a practice that has been going on since the 1970s, providing a wide range of retinal pigment epithelial culture protocols, each producing cells and tissue of varying degrees of similarity to natural retinal pigment epithelium. The purpose of this review is to provide researchers with a ready list of retinal pigment epithelial protocols, their effects on cultured tissue, and their specific possible applications. Protocols using human and animal retinal pigment epithelium cells, derived from tissue or cell lines, are discussed, and recommendations for future researchers included.