Host Alternation of Chikungunya Virus Increases Fitness while Restricting Population Diversity and Adaptability to Novel Selective Pressures
Host Alternation of Chikungunya Virus Increases Fitness while Restricting Population Diversity and Adaptability to Novel Selective Pressures
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DOI：
10.1128/jvi.01918-10
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Vignuzzi, Marco
中科院分区：
文献类型：
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作者：
Coffey, Lark L.;Vignuzzi, Marco
包括基孔肯雅病毒（CHIKV）在内的RNA虫媒病毒进化和维持感染脊椎动物和无脊椎动物宿主的能力的机制尚不清楚。为了了解宿主特异性如何塑造虫媒病毒群体，我们研究了CHIKV群体在无脊椎动物和脊椎动物细胞（无脊椎脊椎动物）之间交替传代以模拟自然交替，并将结果与通过传代在单细胞类型中人工释放的群体的结果进行了对比。这些CHIKV群体通过测量遗传多样性、适应性变化和对新选择压力的适应性来表征。交替传代的适宜度增加最大，种群多样性变化不大。遗传多样性的增加在连续传代后最大，并与更强的适应性相关。这些结果表明，在维持无脊椎动物细胞循环的适应性之间存在一种进化权衡，只有通过增强种群多样性和广泛探索序列空间才能实现最大的适应性。
The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate and vertebrate cells (invertebrate vertebrate) to simulate natural alternation and contrasted the results with those for populations that were artificially released from cycling by passage in single cell types. These CHIKV populations were characterized by measuring genetic diversity, changes in fitness, and adaptability to novel selective pressures. The greatest fitness increases were observed in alternately passaged CHIKV, without drastic changes in population diversity. The greatest increases in genetic diversity were observed after serial passage and correlated with greater adaptability. These results suggest an evolutionary trade-off between maintaining fitness for invertebrate vertebrate cell cycling, where maximum adaptability is possible only via enhanced population diversity and extensive exploration of sequence space.