Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial.
Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial.
复制标题
使用脱细胞胰腺支架体外培养源自胰腺 β 样细胞的 iPSC：初步试验
DOI：
10.1155/2017/4276928
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发表时间：
2017
影响因子：
--
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Wan J;Huang Y;Zhou P;Guo Y;Wu C;Zhu S;Wang Y;Wang L;Lu Y;Wang Z
糖尿病是一种疾病，2015年影响了4.15亿患者。为了替代对移植的显著需求和与移植相关的发病率，评估了从诱导多能干细胞（iPSC）分化的β样细胞的产生。这种方法与具有天然细胞外基质（ECM）和理想的立方体环境的有前途的脱细胞支架相关，这将促进细胞在体内的生长。我们的工作集中于将脱细胞的大鼠胰腺支架与小鼠GFP+-iPSC衍生的胰腺β样细胞结合，以评估脱细胞支架是否可以促进β样细胞的生长和功能。从GFP+-iPSC分化β样细胞，并通过在动态循环灌注装置中培养来评估。我们的研究结果表明，脱细胞胰腺支架显示出良好的生化特性。此外，与基于平板的细胞培养物相比，支架不仅可以支持β样细胞的存活，而且还加速了胰岛素的表达。总之，这些结果表明，脱细胞胰腺支架可以为β样细胞的细胞活性（包括存活和胰岛素分泌）提供合适的平台。该研究为脱细胞支架与iPSCs衍生的β样细胞结合再生胰岛素分泌器官作为潜在的临床应用提供了初步支持。
Diabetes mellitus is a disease which has affected 415 million patients in 2015. In an effort to replace the significant demands on transplantation and morbidity associated with transplantation, the production of β-like cells differentiated from induced pluripotent stem cells (iPSCs) was evaluated. This approach is associated with promising decellularized scaffolds with natural extracellular matrix (ECM) and ideal cubic environment that will promote cell growth in vivo. Our efforts focused on combining decellularized rat pancreatic scaffolds with mouse GFP+-iPSCs-derived pancreatic β-like cells, to evaluate whether decellularized scaffolds could facilitate the growth and function of β-like cells. β-like cells were differentiated from GFP+-iPSCs and evaluated via cultivating in the dynamic circulation perfusion device. Our results demonstrated that decellularized pancreatic scaffolds display favorable biochemical properties. Furthermore, not only could the scaffolds support the survival of β-like cells, but they also accelerated the expression of the insulin as compared to plate-based cell culture. In conclusion, these results suggest that decellularized pancreatic scaffolds could provide a suitable platform for cellular activities of β-like cells including survival and insulin secretion. This study provides preliminary support for regenerating insulin-secreting organs from the decellularized scaffolds combined with iPSCs derived β-like cells as a potential clinical application.