Murine Th1 and Th2 clones proliferate optimally in response to distinct antigen-presenting cell populations.
Murine Th1 and Th2 clones proliferate optimally in response to distinct antigen-presenting cell populations.
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DOI：
10.4049/jimmunol.146.6.1750
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发表时间：
1991-03
影响因子：
4.4
通讯作者：
T. Gajewski;M. Pinnas;T. Wong;F. Fitch
中科院分区：
文献类型：
--
作者：
T. Gajewski;M. Pinnas;T. Wong;F. Fitch
我们最近已经设计了一种方法，用于从相同的致敏淋巴结细胞制备物中获得OVA特异性Th 1和Th 2克隆。使用一组这样的细胞，我们已经检查了不同的APC群体刺激Th 1和Th 2克隆增殖的能力。两个亚群增殖良好，在整个脾细胞的存在下，响应于OVA。然而，纯化的B细胞刺激最佳增殖的Th 2克隆，而贴壁细胞刺激最佳增殖的Th 1克隆。用3300 rad照射的脾细胞刺激的Th 2细胞的增殖反应显著低于用1000 rad处理的脾细胞所观察到的增殖反应; Th 1克隆对暴露于任一照射剂量的脾细胞的反应类似。Th 1和Th 2克隆的差异活化与MHC限制性元件或对针对CD 4或LFA-1的mAb抑制的敏感性无关。在次优增殖条件下，每个亚群的嗜银蛋白产生仍然发生，表明适当的Ag加工和呈递事件已经发生。使用不需要处理的特定OVA肽观察到相同的响应模式，表明Th 1和Th 2克隆对不同APC群体的差异响应性不是有缺陷的Ag处理的结果。rIL-1和rIL-6都不能恢复任一亚群的最佳增殖。我们的研究结果表明，独特的辅因子是必要的最佳增殖的Th 1和Th 2克隆，这些辅因子是由专门的APC群体。
We recently have devised a method for the derivation of OVA-specific Th1 and Th2 clones from the same primed lymph node cell preparation. Using a panel of such cells, we have examined the ability of distinct APC populations to stimulate proliferation of Th1 and Th2 clones. Both subsets proliferated well in response to OVA in the presence of whole spleen cells. However, purified B cells stimulated optimal proliferation of Th2 clones, whereas adherent cells stimulated optimal proliferation of Th1 clones. The proliferative response of Th2 cells stimulated with spleen cells irradiated with 3300 rad was dramatically less than that observed in response to spleen cells treated with 1000 rad; Th1 clones responded similarly to spleen cells exposed to either irradiation dose. Differential activation of Th1 and Th2 clones did not correlate with MHC-restricting element, or susceptibility to inhibition by mAb directed against CD4 or LFA-1. Lymphokine production by each subset still occurred under conditions of suboptimal proliferation, suggesting that the appropriate Ag processing and presentation events had transpired. The same pattern of response was observed using a specific OVA peptide that does not require processing, suggesting that differential responsiveness of Th1 and Th2 clones to different APC populations is not a result of defective Ag processing. Neither rIL-1 nor rIL-6 restored optimal proliferation of either subset. Our results suggest that unique cofactors are necessary for the optimal proliferation of Th1 and Th2 clones, and that these cofactors are produced by specialized APC populations.