Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection.
Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection.
复制标题
DOI：
10.1016/j.chroma.2006.06.101
复制
发表时间：
2006-08
期刊：
影响因子：
--
通讯作者：
K. Tsukagoshi;Shingo Ishida;Yuichi Oda;K. Noda;R. Nakajima
中科院分区：
文献类型：
--
作者：
K. Tsukagoshi;Shingo Ishida;Yuichi Oda;K. Noda;R. Nakajima
我们研制了一种紧凑型聚四氟乙烯（PTFE）组装式毛细管电泳化学发光（CL）检测系统。采用鲁米诺-微过氧化物酶-过氧化氢化学发光反应。该器械为矩形（60 mm × 40 mm × 30 mm），包括三个带电极的储液器（样品、迁移缓冲液和检测储液器）。检测容器包括光纤，以将毛细管尖端处的光传输到光电倍增管。异硫氰酸异鲁米诺（ILITC）作为一个模型进行分析，使用该装置与熔融石英或聚四氟乙烯毛细管长度为10厘米。我们还使用样品池作为反应器，用于固定在玻璃珠上的抗人血清白蛋白和异硫氰酸异鲁米诺标记的人血清白蛋白之间的免疫反应。本发明的具有毛细管的聚四氟乙烯组件可用作用于分离和检测的手掌大小的分析装置以及反应器。
We have developed a compact polytetrafluoroethylene (PTFE) assembly-type capillary electrophoresis with chemiluminescence (CL) detection system. Luminol–microperoxidase–hydrogen peroxide chemiluminescence reaction was adopted. The device is rectangular in shape (60mm×40mm×30mm) and includes three reservoirs (sample, migration buffer, and detection reservoirs) with electrodes. The detection reservoir includes an optical fiber to transport light at the capillary tip to a photomultiplier tube. Isoluminol isothiocyanate (ILITC) was analyzed as a model using this device with fused-silica or polytetrafluoroethylene capillary tubes 10cm in length. We also used the sample reservoir as a reactor for an immune reaction between anti-human serum albumin immobilized on glass beads and isoluminol isothiocyanate-labeled human serum albumin. The present polytetrafluoroethylene assembly with the capillary tube was useful as a palm-sized analysis device for separation and detection, as well as a reactor.