Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord.
Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord.
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DOI：
10.1016/j.bbrc.2006.09.088
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发表时间：
2006-11
影响因子：
3.1
通讯作者：
Miho Sato;K. Nakahara;S. Goto;H. Kaiya;M. Miyazato;Y. Date;M. Nakazato;K. Kangawa;N. Murakami
中科院分区：
文献类型：
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作者：
Miho Sato;K. Nakahara;S. Goto;H. Kaiya;M. Miyazato;Y. Date;M. Nakazato;K. Kangawa;N. Murakami
用RT-PCR和免疫组化方法检测生长激素促分泌素受体（GHS-R）mRNA及其蛋白在大鼠胚胎脊髓组织中的表达。在体外，超过3 nM的ghrelin和des-acyl ghrelin诱导胎儿脊髓原代培养细胞的显着增殖。然后使用神经元前体标记物巢蛋白和细胞增殖标记物5-溴-2 ′-脱氧尿苷（BrdU）的抗体对增殖细胞进行双重染色，并且还发现巢蛋白阳性细胞与抗GHS-R的抗体共染色。此外，使用[125 I]脱酰基生长素释放肽的结合研究表明存在脱酰基生长素释放肽的特异性结合位点，并证实该结合被未标记的脱酰基生长素释放肽或生长素释放肽取代。这些结果表明，生长素释放肽和脱酰基生长素释放肽诱导神经元前体细胞的增殖，这是既依赖和独立的GHS-R，这表明生长素释放肽和脱酰基生长素释放肽参与胎儿脊髓的神经发生。
Expressions of the growth hormone secretagogue receptor (GHS-R) mRNA and its protein were confirmed in rat fetal spinal cord tissues by RT-PCR and immunohistochemistry. In vitro, over 3nM ghrelin and des-acyl ghrelin induced significant proliferation of primary cultured cells from the fetal spinal cord. The proliferating cells were then double-stained using antibodies against the neuronal precursor marker, nestin, and the cell proliferation marker, 5-bromo-2′-deoxyuridine (BrdU), and the nestin-positive cells were also found to be co-stained with antibody against GHS-R. Furthermore, binding studies using [125I]des-acyl ghrelin indicated the presence of a specific binding site for des-acyl ghrelin, and confirmed that the binding was displaced with unlabeled des-acyl ghrelin or ghrelin. These results indicate that ghrelin and des-acyl ghrelin induce proliferation of neuronal precursor cells that is both dependent and independent of GHS-R, suggesting that both ghrelin and des-acyl ghrelin are involved in neurogenesis of the fetal spinal cord.