In situ-bound antibodies eluted from the skin of patients with bullous pemphigoid are preferentially directed against the 230-kD bullous pemphigoid antigen.
In situ-bound antibodies eluted from the skin of patients with bullous pemphigoid are preferentially directed against the 230-kD bullous pemphigoid antigen.
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从大疱性类天疱疮患者皮肤洗脱的原位结合抗体优先针对 230-kD 大疱性类天疱疮抗原。
DOI：
10.1111/1523-1747.ep12326613
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Korman,NJ
中科院分区：
文献类型：
--
作者：
Korman,NJ
已鉴定出两种大疱性类天疱疮抗原 (BPA)：一种 230 kD 的细胞内半桥粒相关分子和一种 180 kD 的跨膜半桥粒相关分子。尽管大疱性类天疱疮 (BP) 患者已被证明具有针对一种或两种 BPA 的循环抗体，但组织结合 BP 自身抗体的抗原特异性尚未研究。由于这些皮肤结合抗体可能在疾病发生中发挥重要作用，我们试图确定它们的抗原特异性。用甘氨酸从盐裂病灶周围皮肤活检标本中洗脱原位结合的 BP 抗体，并进行免疫沉淀。 13 名患者中的 10 名具有识别 230-kD BPA 的甘氨酸洗脱抗体，而 13 名患者中的 4 名具有识别 180-kD BPA 的甘氨酸洗脱抗体。当这些甘氨酸洗脱的 BP 皮肤标本依次用尿素洗脱时，我们发现抗体具有相同的特异性，或者对其中一种 BPA 失去了反应性，但对以前用甘氨酸洗脱未检测到的任何 BPA 没有反应性。用十二烷基硫酸钠连续洗脱显示没有检测到 BP 抗体。循环、组织结合和洗脱的 BP 抗体的重链和轻链同种型非常相似，表明我们没有洗脱 BP 抗体的某些子集，这些患者的血清含有识别一种或两种 BPA 的循环抗体。我们的观察表明，从 BP 患者皮肤洗脱的原位结合抗体优先针对 230-kD BPA。
Two bullous pemphigoid antigens (BPAs) have been identified: a 230-kD intracellular hemidesmosome-associated molecule and a 180-kD transmembrane hemidesmosome-associated molecule, Although patients with bullous pemphigoid (BP) have been shown to have circulating antibodies directed against one or both BPAs, the antigenic specificity of tissue-bound BP autoantibodies has not been studied. Because these skin-bound antibodies may play an important role in disease initiation, we sought to determine their antigenic specificity.In situ-bound BP antibodies were eluted with glycine from salt-split perilesional skin biopsy specimens and subjected to immunoprecipitation. Ten of 13 patients had glycine-eluted antibodies that recognized the 230-kD BPA, whereas four of 13 patients had glycine-eluted antibodies that recognized the 180-kD BPA. When these glycine-eluted BP skin specimens were sequentially eluted with urea, we found either antibodies of the same specificity or loss of reactivity to one of the BPAs, but no reactivity to any BPAs previously undetected with glycine elution. Sequential elution with sodium dodecylsulfate revealed no detectable BP antibodies. The heavy- and light-chain isotypes of the circulating, tissue-bound, and eluted BP antibodies were very similar, suggesting that we are not eluting certain subsets of BP antibodies, Sera from these patients contained circulating antibodies that recognized either one or both BPAs. Our observations demonstrate thatin situ-bound antibodies eluted from the skin of patients with BP are preferentially directed against the 230-kD BPA.