The separation and amino acid analysis of collagen crosslinks on an extended basic ion-exchange column.
The separation and amino acid analysis of collagen crosslinks on an extended basic ion-exchange column.
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在扩展碱性离子交换柱上胶原交联的分离和氨基酸分析。
DOI：
10.1016/0003-2697(81)90486-3
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发表时间：
1981
影响因子：
2.9
通讯作者：
Tanzer,ML
中科院分区：
文献类型：
--
作者：
Housley,TJ;Tanzer,ML
在扩展的碱性氨基酸分析柱上分离和分析了胶原蛋白中发现的主要可还原交联。与茚三酮反应可以直接分析胶原交联，包括羟基羟醛-组氨酸，这是一种天然存在的不可还原的交联。除了已知的交联之外，对组织水解产物的直接氨基酸分析表明，在 NaB3H4 还原和未还原的胶原组织中都存在未知的茚三酮反应成分。 Bio-Gel P-2 凝胶过滤柱上水解产物的初始分级可部分分离氨基酸和交联，从而能够更直接地分析样品中存在的交联，并检测潜在的新交联。结果还表明，在 NaB3H4 还原之前，牛皮肤和骨骼中通常存在大量已知的交联。
The major reducible crosslinks found in collagen were separated and analyzed on an extended basic amino acid analyzer column. Reaction with ninhydrin allows the direct analysis of collagen crosslinks, including hydroxyaldol-histidine, a naturally occurring, nonreducible crosslink. In addition to known crosslinks, direct amino acid analysis of tissue hydrolysates reveals the presence of an unknown, ninhydrin-reactive component, in both NaB3H4-reduced and unreduced collagenous tissues. Initial fractionation of hydrolysates on a Bio-Gel P-2 gel filtration column provides partial separaton of amino acids and crosslinks and enables more direct analysis of the crosslinks present in the samples, as well as detecting potential new crosslinks. The results also show that, prior to NaB3H4reduction, substantial amounts of known crosslinks are normally present in bovine skin and bone.