Plasma glucagon levels measured by sandwich ELISA are correlated with impaired glucose tolerance in type 2 diabetes
Plasma glucagon levels measured by sandwich ELISA are correlated with impaired glucose tolerance in type 2 diabetes
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DOI：
10.1507/endocrj.ej20-0079
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发表时间：
2020-01-01
影响因子：
2
通讯作者：
Kitamura, Tadahiro
中科院分区：
文献类型：
--
作者：
Kobayashi, Masaki;Satoh, Hiroaki;Kitamura, Tadahiro
胰升糖素功能障碍和胰岛素功能障碍与2型糖尿病的发病机制有关。然而，目前尚不清楚血浆胰高血糖素水平的测定是否有助于了解T2 DM的病理生理机制。我们最近报道，在健康受试者中，夹心ELISA法比常规RIA法提供更准确的血浆胰高血糖素水平。本研究采用双抗体夹心ELISA法和放射免疫法测定血浆胰高血糖素水平，比较T2 DM患者和健康受试者在口服葡萄糖(OGTT)或餐耐量试验(MTT法)中的变化。证实双抗体夹心ELISA法较RIA法更能检测出健康人与T2 DM患者空腹血糖水平及血浆胰高血糖素反应动力学的差异。我们还发现下列参数有显著差异：(1)空腹高血糖素，(2)OGTT中高血糖素曲线下面积(AUC)，(3)OGTT或MTT中0-30min内高血糖素值的变化(0-0.5h)。其中，差异性最明显的是Delta Glucagon(0~0.5h)的MTT值。当我们将T2 DM患者分为两组，在MTT值低于或高于最大值的情况下，糖尿病患者的糖耐量受损更明显。这些结果提示，用双抗体夹心ELISA法测定血浆胰高血糖素水平有助于加深对T2 DM病理生理机制的认识。
Glucagon dysfunction as well as insulin dysfunction is associated with the pathogenesis of type 2 diabetes (T2DM). However, it is still unclear whether the measurement of plasma glucagon levels is useful in understanding the pathophysiology of T2DM. We recently reported that sandwich ELISA provides more accurate plasma glucagon values than conventional RIA in healthy subjects. Here we used sandwich ELISA as well as RIA to assess plasma glucagon levels, comparing them in T2DM patients and healthy subjects during oral glucose (OGTT) or meal tolerance tests (MTT). We confirmed that sandwich ELISA was able to detect more significant difference between healthy subjects and T2DM patients in the fasting levels and the response dynamics of plasma glucagon than RIA. We also found significant differences in the following glucagon parameters: (1) fasting glucagon, (2) the area under the curve (AUC) of glucagon in OGTT, and (3) the change in glucagon between 0 and 30 min (Delta Glucagon(0-0.5h)) in OGTT or MTT. Among these, the most apparent difference was Delta Glucagon(0-0.5h) in MTT. When we divided T2DM patients into two groups whose Delta Glucagon(0-0.5h) in MTT was either below or above the maximum value in healthy subjects, the group with higher Delta Glucagon(0-0.5h) showed more significant impairment of glucose tolerance. These results suggest that the assessment of plasma glucagon levels by sandwich ELISA might enhance our understanding of the pathophysiology of T2DM.