Th2 cell-derived histamine is involved in nasal Th2 infiltration in mice
Th2 cell-derived histamine is involved in nasal Th2 infiltration in mice
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DOI：
10.1007/s00011-021-01458-x
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发表时间：
2021-04-03
影响因子：
6.7
通讯作者：
Tokunaga, Fuminori
中科院分区：
文献类型：
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作者：
Iwasaki, Naruhito;Terawaki, Seigo;Tokunaga, Fuminori
目的肥大细胞和嗜碱性粒细胞分泌的组胺在变态反应中起重要作用。虽然T细胞也产生组胺，但T细胞产生的组胺的参与仍然是个谜。我们试图揭示的作用，辅助性T细胞2（Th2）细胞衍生的组胺在鼻过敏性疾病。方法采用酶免疫法（EIA）检测Th2细胞组胺分泌。实时荧光定量PCR检测HDC mRNA的表达。为了研究Th2细胞来源的组胺在体内的作用，我们分析了抗原特异性Th2细胞转移小鼠模型。结果Th2细胞通过T细胞受体刺激产生组胺，这些特性是Th2细胞所特有的，而不是Th1细胞和初始CD4 T细胞所特有的。Th2细胞产生的组胺参与了抗原暴露后Th2细胞的浸润。结论Th2细胞源性组胺在鼻变态反应性疾病中起重要作用。
Objective Histamine derived from mast cells and basophils plays important roles in inducing allergic symptoms. Although T cells also produce histamine, the involvement of the histamine produced from T cells has remained enigmatic. We sought to reveal the roles of T helper 2 (Th2) cell-derived histamine in nasal allergic disorders. Methods The histamine production from Th2 cells was measured by EIA. The mRNA expression of histidine decarboxylase (HDC) was measured by real-time PCR. To investigate the roles of Th2 cell-derived histamine in vivo, we analyzed an antigen-specific Th2 cell transfer mouse model. Results Th2 cells produced histamine by T cell receptor stimulation, and these properties were specific for Th2 cells, but not Th1 cells and naive CD4 T cells. The histamine produced from Th2 cells was involved in the infiltrations of Th2 cells in response to antigen exposure. Conclusion These results suggest that Th2 cell-derived histamine play important roles in nasal allergic disorders.