Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles
Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles
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DOI：
10.1016/j.febslet.2015.11.036
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发表时间：
2015-12-21
期刊：
影响因子：
3.5
通讯作者：
Ochiya, Takahiro
中科院分区：
文献类型：
--
作者：
Hagiwara, Keitaro;Katsuda, Takeshi;Ochiya, Takahiro
细胞外囊泡（EV）含有微小RNA（miRNA）。然而，在EV中募集miRNA的确切分子机制尚未得到很好的表征。基于蛋白质组学分析，我们鉴定了膜联蛋白A2（ANXA 2）的沉默显著降低了EV中miRNA的量。此外，微阵列分析显示ANXA 2以序列非依赖性方式调节miRNA加载到EV中。最后，免疫沉淀分析证实，ANXA 2可以在Ca 2+存在下结合EV中的miRNA。这些观察结果表明，ANXA 2在miRNA包装成EV的过程中起着重要作用。(C)2015作者由Elsevier B.V.代表欧洲生物化学学会联合会出版。
Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence independent manner. Lastly, immunoprecipitation analysis confirmed that ANXA2 could bind miRNAs in EVs in the presence of Ca2+. These observations demonstrate that ANXA2 plays an important role in the packaging process of miRNAs into EVs. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.