Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19
Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19
复制标题
COVID-19 患者支气管肺泡免疫细胞的单细胞景观。
DOI：
10.1038/s41591-020-0901-9
复制
发表时间：
2020-05-12
期刊：
影响因子：
82.9
通讯作者：
Zhang, Zheng
中科院分区：
文献类型：
--
作者：
Liao, Mingfeng;Liu, Yang;Zhang, Zheng
与2019冠状病毒病（COVID-19）严重程度相关的呼吸道免疫特征目前尚不清楚。我们通过使用单细胞RNA测序来表征来自不同严重程度的COVID-19患者和健康人的支气管肺泡灌洗液免疫细胞。严重COVID-9患者的支气管肺泡灌洗液中富含促炎性单核细胞衍生的巨噬细胞。中度病例的特征是存在高度克隆扩增的CD 8（+）T细胞。支气管肺泡免疫微环境图谱提示了COVID-19发病和恢复的潜在机制。支气管肺泡灌洗液的单细胞转录组和T细胞受体分析提示，重度COVID-19患者中促炎性巨噬细胞富集，中度COVID-19患者中存在克隆扩增的CD 8（+）T细胞。
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8(+) T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8(+) T cells in patients with moderate COVID-19.