Analysis of retention of gadolinium by brain, bone, and blood following linear gadolinium-based contrast agent administration in rats with experimental sepsis
Analysis of retention of gadolinium by brain, bone, and blood following linear gadolinium-based contrast agent administration in rats with experimental sepsis
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DOI：
10.1002/mrm.28060
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发表时间：
2019-11-01
影响因子：
3.3
通讯作者：
Morton, Kathryn A.
中科院分区：
文献类型：
--
作者：
Damme, Nikolas M.;Fernandez, Diego P.;Morton, Kathryn A.
目的识别可能易受MRI造影剂所致钆（Gd）脑沉积影响的人群非常重要。在注射线性Gd造影剂后24小时至6周的时间间隔内，将对照大鼠和实验性内毒素诱导的脓毒症大鼠（导致神经炎症和血脑屏障破坏）的脑、血液和骨中Gd含量进行比较。方法雄性大鼠腹腔注射脂多糖10 mg/kg。对照动物未接受注射。24小时后，静脉注射0.2 mmol/kg钆贝葡胺。通过电感耦合等离子体质谱法在24小时、1周、3周和6周测量脑、血液和骨Gd水平。结果脂多糖组大鼠血Gd在24 h至1周内迅速下降，此后检测不到，与对照组相比无显著性差异。在所有时间点，脂多糖组的脑Gd水平显著高于对照组大鼠（4.29-2.36倍），骨水平略高于对照组大鼠（1.35-1.11倍），6周时有显著保留。结论实验性脓毒症可导致Gd在大鼠脑和骨组织中沉积增加。虽然血Gd清除迅速，脑和骨保留大量的Gd，即使在6周后造影剂注射。
Purpose It is important to identify populations that may be vulnerable to the brain deposition of gadolinium (Gd) from MRI contrast agents. At intervals from 24 hours to 6 weeks following injection of a linear Gd contrast agent, the brain, blood and bone content of Gd were compared between control rats and those with experimental endotoxin-induced sepsis that results in neuroinflammation and blood-brain barrier disruption. Methods Male rats were injected intraperitoneally with 10 mg/kg lipopolysaccharide. Control animals received no injection. Twenty-four hours later, 0.2 mmol/kg of gadobenate dimeglumine was injected intravenously. Brain, blood, and bone Gd levels were measured at 24 hours, 1 week, 3 weeks, and 6 weeks by inductively coupled plasma mass spectroscopy. Results Blood Gd decreased rapidly between 24 hours and 1 week, and thereafter was undetectable, with no significant difference between lipopolysaccharide and control rats. Brain levels of Gd were significantly higher (4.29-2.36-fold) and bone levels slightly higher (1.35-1.11-fold) in lipopolysaccharide than control rats at all time points with significant retention at 6 weeks. Conclusion Experimental sepsis results in significantly higher deposition of Gd in the brain and bone in rats. While blood Gd clears rapidly, brain and bone retained substantial Gd even at 6 weeks following contrast injection.