The genome and transcriptome of Phalaenopsis yield insights into floral organ development and flowering regulation.
The genome and transcriptome of Phalaenopsis yield insights into floral organ development and flowering regulation.
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作者：
Huang JZ;Lin CP;Cheng TC;Huang YW;Tsai YJ;Cheng SY;Chen YW;Lee CP;Chung WC;Chang BC;Chin SW;Lee CY;Chen FC
蝴蝶兰是一种重要的盆栽花卉，在世界各地具有很高的经济价值。我们报道了一个重要的冬季开花蝴蝶兰品种‘KHM190’的3.1 GB草稿基因组组装。我们生成了89.5 GB的RNA-seq和1.13亿个sRNA-seq读数，以使用这些数据来识别41,153个蛋白质编码基因和188个miRNA家族。我们还通过重新测序获得了夏季开花物种蝴蝶兰‘B8802’的基因组草图。通过比较两个蝴蝶兰品种的基因组数据，可以鉴定出691,532个单核苷酸多态。在本研究中，我们揭示了PhAGL6b在调节唇器发育中的关键作用涉及到大嘴唇突变体中的选择性剪接。花瓣或萼片过表达PhAGL6b会导致转化为唇状结构。我们还发现，在生殖期转变过程中，调节开花时间基因表达的赤霉素途径是受低温诱导的。因此，我们的工作为蝴蝶兰的开花控制、花卉构型开发和育种提供了宝贵的资源。
The Phalaenopsis orchid is an important potted flower of high economic value around the world. We report the 3.1 Gb draft genome assembly of an important winter flowering Phalaenopsis ‘KHM190’ cultivar. We generated 89.5 Gb RNA-seq and 113 million sRNA-seq reads to use these data to identify 41,153 protein-coding genes and 188 miRNA families. We also generated a draft genome for Phalaenopsis pulcherrima ‘B8802,’ a summer flowering species, via resequencing. Comparison of genome data between the two Phalaenopsis cultivars allowed the identification of 691,532 single-nucleotide polymorphisms. In this study, we reveal that the key role of PhAGL6b in the regulation of labellum organ development involves alternative splicing in the big lip mutant. Petal or sepal overexpressing PhAGL6b leads to the conversion into a lip-like structure. We also discovered that the gibberellin pathway that regulates the expression of flowering time genes during the reproductive phase change is induced by cool temperature. Our work thus depicted a valuable resource for the flowering control, flower architecture development, and breeding of the Phalaenopsis orchids.