Thermally-induced protein-polyphenol co-assemblies: beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG
Thermally-induced protein-polyphenol co-assemblies: beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG
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DOI：
10.1016/j.foodhyd.2010.03.015
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发表时间：
2010-11-01
影响因子：
10.7
通讯作者：
Livney, Yoav D.
中科院分区：
文献类型：
--
作者：
Shpigelman, Avi;Israeli, Gal;Livney, Yoav D.
在食品中传递敏感的水溶性化合物是一个重要的挑战。 (-)-表没食子儿茶素-3-没食子酸酯 (EGCG) 是绿茶中的主要儿茶素，是一种有效的抗氧化剂，具有多种健康益处。然而，它对氧化的敏感性限制了它在预防医学饮食中的富集。我们研究了使用热改性 β-乳球蛋白 (β-Lg) 形成共组装纳米载体来递送 EGCG 的可能性，作为通过热改性蛋白质递送多酚的模型系统。使用荧光光谱、DLS 和分光光度法，我们发现，在冷却和涡旋期间将 EGCG 添加到预热（75-85 摄氏度，20 分钟）的 β-Lg 溶液中时，可以获得最佳的纳米包封。测得的与加热蛋白质的关联常数为3.7 x 10(5) M-1，比与天然蛋白质的关联常数：1.05 x 10(5) M-1高约3.5倍。热诱导蛋白-EGCG共组装体小于50 nm，因此保持了优异的透明度，使其能够在透明饮料中应用。这些复合物为 EGCG 提供了相当大的保护，使其免受氧化降解：与未受保护的 EGCG 相比，纳米包埋的 EGCG 的初始降解率低 33 倍，并且在 8 天内的降解速度慢 3.2 倍。 (C) 2010 Elsevier Ltd. 保留所有权利。
Delivery of sensitive water-soluble compounds in foods is an important challenge. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin in green tea, is a potent antioxidant with numerous attributed health benefits. However, its sensitivity to oxidation limits its enrichment in the diet for preventive medicine. We have studied the possibility of using thermally modified beta-lactoglobulin (beta-Lg) to form co-assembled nanovehicles for delivery of EGCG, as a model system for delivery of polyphenols by heat-modified proteins. Using fluorescence spectroscopy, DLS and spectrophotometry we found that optimal nano-entrapment is obtained when EGCG is added to preheated (75-85 degrees C, 20 min) beta-Lg solution during cooling and vortexing. The measured association constant with the heated protein was 3.7 x 10(5) M-1, about 3.5-fold higher than that with the native protein: 1.05 x 10(5) M-1. Thermally-induced protein-EGCG co-assemblies were smaller than 50 nm, thus excellent transparency was maintained, enabling their application in clear beverages. These complexes conferred considerable protection to EGCG against oxidative degradation: A 33-fold lower initial degradation rate, and a 3.2-fold slower degradation over 8 days were observed for nano-entrapped compared to unprotected EGCG. (C) 2010 Elsevier Ltd. All rights reserved.