[Experimental study on pathophysiology and treatment of congenital hydrocephalus evaluated by magnetic resonance imaging and magnetic resonance spectroscopy].
[Experimental study on pathophysiology and treatment of congenital hydrocephalus evaluated by magnetic resonance imaging and magnetic resonance spectroscopy].
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磁共振成像和磁共振波谱评估先天性脑积水病理生理学和治疗的实验研究
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发表时间：
1992
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通讯作者：
J. Minamikawa
中科院分区：
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作者：
J. Minamikawa
一、先天性脑积水病理生理学的实验研究众所周知，脑积水的主要发病机制是脑脊液循环障碍。为此，许多关于脑积水的研究都是从脑脊液循环的角度进行的，无论是实验还是临床。然而，在体脑积水的形态变化与脑能量代谢变化之间的相关性研究尚不多见。因此，本研究从实验上探讨了先天性脑积水大鼠脑组织形态变化与脑能量代谢变化的相关性。用磁共振成像(MRI)评价脑组织的形态变化，并测量顶区脑组织的纵向松弛时间(T1)。用31P磁共振波谱(31P-MRS)法检测脑组织能量代谢，用31P-MRS法检测脑磷脂膜代谢。产生31P谱的感兴趣区(ROI)位于前脑和顶区。用PCR/PI比值作为细胞生物能量状态的指标。以PME/β-ATP比值和PDE/β-ATP比值作为脑磷脂膜代谢的指标。用31P-MRS法测定细胞内pH。选用HTX系先天性脑积水大鼠50只。动物分为非脑积水组(n=15)和脑积水组(n=35)。脑积水组大鼠按脑积水程度分为轻度组(n=15)、中度组(n=10)和重度组(n=10)。实验结果如下：1)轻、中、重度脑积水组大鼠T1值较非脑积水组显著延长(P<0.01)，提示大脑皮层间质水肿扩大。2)评价T1值与脑血管阻力的相关性，相关系数r=0.932，表明两者具有较高的相关性。3)轻、中、重度脑积水组大鼠的Pcr/PI比值较非脑积水组显著降低(p<0.01)，表明先天性脑积水大鼠存在脑能量代谢障碍。4)PCR/PI比值可作为预测先天性脑积水预后的指导性指标。
I. Experimental Study on Pathophysiology of Congenital Hydrocephalus It is well known that the major pathogenic mechanism of hydrocephalus is disturbance in cerebrospinal fluid (CSF) circulation. For this reason, many studies on hydrocephalus were intended from the viewpoint of CSF circulation both experimentally and clinically. However, few studies have yet been done on the correlation between the morphological changes and the changes in cerebral energy metabolism in hydrocephalus in vivo. So, in this study, the correlation between the morphological changes and the changes in cerebral energy metabolism in congenital hydrocephalic rats was evaluated experimentally. The morphological changes were estimated by using magnetic resonance imaging (MRI), and the longitudinal relaxation time (T1) of brain tissue at parietal area was also measured. The cerebral energy metabolism was evaluated by using 31P magnetic resonance spectroscopy (MRS) method, and cerebral phospholipid membrane metabolism was also evaluated by using 31P-MRS method. The region of interest (ROI) giving rise to the 31P spectra was placed at fore-brain and parietal area. The PCr/Pi ratio was used as the chosen indicator of cellular bioenergetic status. The PME/beta-ATP ratio and PDE/beta-ATP ratio were used as the chosen indicator of cerebral phospholipid membrane metabolism. The intracellular pH was also evaluated by using 31P-MRS method. Fifty congenital hydrocephalic rats of the HTX strain were used. The animals were divided into two groups--non-hydrocephalic group (n = 15) and hydrocephalic group (n = 35)--. The rats of hydrocephalic group were subdivided into three smaller groups according to the degree of hydrocephalus--mild (n = 15), moderate (n = 10) and severe (n = 10)--, which was estimated by using the cerebro-ventricular ratio (CVR) in coronal section of MRI. Experimental results were as follows: 1) The T1 values in rats of mild, moderate and severe hydrocephalic groups showed significant elongation in comparison with the value in non-hydrocephalic group (p less than 0.01), which indicated the expansion of interstitial edema in cerebral cortex. 2) The correlation between the T1 value and the CVR was evaluated and the correlation coefficient (r) was 0.932 which indicated high correlation. 3) The PCr/Pi ratios in rats of mild, moderate and severe hydrocephalic groups were decreased significantly in comparison with the value in non-hydrocephalic group (p less than 0.01), which demonstrated the disturbance of cerebral energy metabolism in congenital hydrocephalic rats. 4) The PCr/Pi ratio seemed to give the indicative data concerning the prognosis of congenital hydrocephalus.(ABSTRACT TRUNCATED AT 400 WORDS)