MEASUREMENT OF DNA LENGTH BY GEL-ELECTROPHORESIS
MEASUREMENT OF DNA LENGTH BY GEL-ELECTROPHORESIS
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DOI：
10.1016/0003-2697(79)90235-5
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
SOUTHERN, EM
中科院分区：
文献类型：
--
作者：
SOUTHERN, EM
将片段长度与迁移率的倒数作图，比传统的半对数图在更宽的范围内得到直线。可以通过简单的校正来消除曲率。本文还给出了由迁移率直接计算分子量的方法。
Plotting fragment length against reciprocal of mobility gives a straight line over a wider range than the conventional semilogarithmic plot. Curvature can be removed by a simple correction. A method is also given for determining MW from mobilities by direct calculation.