Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis
Effect of bovine somatotropin on neutrophil functions and clinical symptoms during Streptococcus uberis mastitis
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DOI：
10.3168/jds.s0022-0302(99)75374-9
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发表时间：
1999-07-01
影响因子：
3.5
通讯作者：
Hard, DL
中科院分区：
文献类型：
--
作者：
Hoeben, D;Burvenich, C;Hard, DL
研究了重组牛生长激素（bST）对分离的多形核白细胞的化学发光、脱落和粘附受体（CD11a、CD11b、CD18）表达的影响。同时监测血浆中胰岛素样生长因子- i （IGF-I）、bST、皮质醇和α -乳清蛋白的浓度。此外，还研究了奶牛实验诱导的ubercoccus ubertis乳腺炎的一般和局部临床症状以及循环白细胞的分化。10头奶牛在左舍感染了500 cfu的uberis O140J。5头奶牛在感染前后7 d皮下注射重组bST 500 mg，对照组5头奶牛注射该赋形剂。一般症状（发烧、心动过速、食欲不振和抑郁）和局部症状（肿胀、疼痛、僵硬和牛奶中有斑点）在对照奶牛中更为急性、严重和持久。bST对循环多形核白细胞的化学发光和渗出没有影响，对粘附受体的表达没有影响。重组bST诱导血浆中IGF-I和bST浓度显著升高。经bst处理的奶牛感染后白细胞减少较不明显，循环带中性粒细胞和化髓细胞数量显著降低。两组间皮质醇浓度无显著差异，但α -乳清蛋白血药浓度从感染后6 d开始显著升高。这些结果表明，重组bST治疗通过保护奶牛在随后的uberis乳腺炎中免受严重的局部和一般临床症状，改善了动物福利，但它对循环多形核白细胞的化学发光、水肿和粘附受体的表达没有影响。
The effect of recombinant bovine somatotropin (bST) on the chemiluminescence, diapedesis, and expression of adhesion receptors (CD11a, CD11b, CD18) of isolated polymorphonuclear leukocytes was studied. The plasma concentrations of insulin-like growth factor-I (IGF-I), bST, cortisol, and alpha-lactalbumin were also monitored. In addition, general and local clinical symptoms and the differentiation of circulating leukocytes were also studied during experimentally induced Streptococcus uberis mastitis in cows. Ten cows were infected with 500 cfu of S. uberis O140J in both left quarters. Five cows were subcutaneously treated with 500 mg of recombinant bST 7 d before and after infection, and 5 control cows received the excipient. General (fever, tachycardia, inappetance, and depression) and local symptoms (swelling, pain, firmness, and flecks in milk) were more acute, severe, and longer-lasting in control cows. Treatment with bST had no effect on chemiluminescence and diapedesis of circulating polymorphonuclear leukocytes and no effect on the expression of adhesion receptors. Recombinant bST induced significantly higher IGF-I and bST concentrations in plasma. The leukopenia observed after infection was less pronounced in the bST-treated cows, and the number of circulating band neutrophils and metamyelocytes was significantly lower in the treated group. The concentration of cortisol did not differ between both groups, but the blood concentration of alpha-lactalbumin significantly increased in both groups from 6 d after infection. These results showed that treatment with recombinant bST improves animal welfare by protecting the cows from severe local and general clinical symptoms during subsequent S. uberis mastitis, but that it has no effect on chemiluminescence, diapedesis, and the expression of adhesion receptors of circulating polymorphonuclear leukocytes.