On the origin of the extracellular action potential waveform:: A modeling study
On the origin of the extracellular action potential waveform:: A modeling study
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DOI：
10.1152/jn.00979.2005
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发表时间：
2006-05-01
影响因子：
2.5
通讯作者：
Buzsáki, G
中科院分区：
文献类型：
--
作者：
Gold, C;Henze, DA;Buzsáki, G
尽管细胞外单元记录通常用于检测峰值发生，但它也具有报告通常被视为动作电位细胞内特征的理论能力。我们通过开发一个模型系统来解决这种理论能力，该模型系统捕获了CA1锥体神经元的实验记录的同时记录的细胞内和细胞外记录。我们使用Holt和Koch的线源近似方法来对单个神经元的尖峰活性产生的细胞外动作电位（EAP）电压进行建模。我们比较了在体内记录的CA1锥体神经元的同时细胞内和细胞外记录，并与对相同细胞的模型预测进行了模型预测，该细胞对相同的细胞进行了重建和模拟的隔室模型。该模型准确地重现了EAP的波形和幅度，尽管很难在细胞内和细胞外波形上同时实现良好的匹配。这表明对EAP波形的核算对整个模型提供了相当大的限制。开发的模型解释了波形如何以及为什么相对于记录的单元格变化。有趣的是，每个细胞的树突形态对EAP波形的影响很小。该模型还表明，不同细胞中离子电流的各种组成反映在EAP的特征中。
Although extracellular unit recording is typically used for the detection of spike occurrences, it also has the theoretical ability to report about what are typically considered intracellular features of the action potential. We address this theoretical ability by developing a model system that captures features of experimentally recorded simultaneous intracellular and extracellular recordings of CA1 pyramidal neurons. We use the line source approximation method of Holt and Koch to model the extracellular action potential (EAP) voltage resulting from the spiking activity of individual neurons. We compare the simultaneous intracellular and extracellular recordings of CA1 pyramidal neurons recorded in vivo with model predictions for the same cells reconstructed and simulated with compartmental models. The model accurately reproduces both the waveform and the amplitude of the EAPs, although it was difficult to achieve simultaneous good matches on both the intracellular and extracellular waveforms. This suggests that accounting for the EAP waveform provides a considerable constraint on the overall model. The developed model explains how and why the waveform varies with electrode position relative to the recorded cell. Interestingly, each cell's dendritic morphology had very little impact on the EAP waveform. The model also demonstrates that the varied composition of ionic currents in different cells is reflected in the features of the EAP.