Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803
Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803
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DOI：
10.1021/pr501275a
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发表时间：
2015-02-01
影响因子：
4.4
通讯作者：
Ge, Feng
中科院分区：
文献类型：
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作者：
Mo, Ran;Yang, Mingkun;Ge, Feng
蓝细菌是地球上已知最古老的生命形式，也是唯一能够进行产氧光合作用的原核生物。集胞藻属（Synechocystis sp. PCC 6803）是一种在光合作用和环境适应性研究中广泛应用的模式蓝藻。翻译后蛋白质修饰赖氨酸乙酰化在真核生物和原核生物中起着重要的调节作用，然而，其在蓝藻中的程度和功能仍然未被探索。在此，我们进行了全球集胞藻乙酰组分析，通过肽预分馏，抗体富集，和高精度的LC-MS/MS分析，确定了776乙酰化位点上的513乙酰化蛋白质，并在功能上将它们归类到一个相互作用的地图，显示它们参与各种生物过程。与以前的报告一致，大部分乙酰化位点存在于参与细胞代谢的蛋白质上。有趣的是，首次发现许多参与光合作用的蛋白质，包括藻蓝蛋白（CpcA，CpcB，CpcC和CpcG）和别藻蓝蛋白（ApcA，ApcB，ApcD，ApcE和ApcF）的亚基，都被赖氨酸乙酰化，这表明赖氨酸乙酰化可能在光合作用过程中发挥调节作用。通过免疫沉淀和Western印迹进一步验证了六个与光合作用和碳代谢相关的乙酰化蛋白。我们的数据提供了第一个全球调查的赖氨酸乙酰化蓝藻，并揭示了以前不受重视的作用赖氨酸乙酰化的光合作用的调节。所提供的数据集可以作为一个重要的资源，赖氨酸乙酰化的功能分析在蓝藻和促进整个代谢网络和光合作用过程中，在这个模式蓝藻的阐明。
Cyanobacteria are the oldest known life form inhabiting Earth and the only prokaryotes capable of performing oxygenic photosynthesis. Synechocystis sp. PCC 6803 (Synechocystis) is a model cyanobacterium used extensively in research on photosynthesis and environmental adaptation. Posttranslational protein modification by lysine acetylation plays a critical regulatory role in both eukaryotes and prokaryotes; however, its extent and function in cyanobacteria remain unexplored. Herein, we performed a global acetylome analysis on Synechocystis through peptide prefractionation, antibody enrichment, and high accuracy LC-MS/MS analysis; identified 776 acetylation sites on 513 acetylated proteins; and functionally categorized them into an interaction map showing their involvement in various biological processes. Consistent with previous reports, a large fraction of the acetylation sites are present on proteins involved in cellular metabolism. Interestingly, for the first time, many proteins involved in photosynthesis, including the subunits of phycocyanin (CpcA, CpcB, CpcC, and CpcG) and allophycocyanin (ApcA, ApcB, ApcD, ApcE, and ApcF), were found to be lysine acetylated, suggesting that lysine acetylation may play regulatory roles in the photosynthesis process. Six identified acetylated proteins associated with photosynthesis and carbon metabolism were further validated by immunoprecipitation and Western blotting. Our data provide the first global survey of lysine acetylation in cyanobacteria and reveal previously unappreciated roles of lysine acetylation in the regulation of photosynthesis. The provided data set may serve as an important resource for the functional analysis of lysine acetylation in cyanobacteria and facilitate the elucidation of the entire metabolic networks and photosynthesis process in this model cyanobacterium.