Proteins tightly bound to DNA in the regions of DNA attachment to the skeletal structures of interphase nuclei and metaphase chromosomes
Proteins tightly bound to DNA in the regions of DNA attachment to the skeletal structures of interphase nuclei and metaphase chromosomes
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间期细胞核和中期染色体骨架结构上 DNA 附着区域中与 DNA 紧密结合的蛋白质
DOI：
10.1016/0092-8674(81)90361-5
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
G. Georgiev
中科院分区：
文献类型：
--
作者：
S. Razin;V. V. Chernokhvostov;Andrej V. Roodyn;I. B. Zbarsky;G. Georgiev
与小鼠 L 细胞 DNA 紧密结合的蛋白质在蛋白质骨架的 DNA 附着区域和 DNA 环中进行了检查。通过温和的核酸酶处理分离出含有 2% 至 15% 原始 DNA 的间期核基质和中期染色体支架，以及从骨架中释放的物质。至少有六种对肌糖基-Cs2S04 处理具有抗性的蛋白质仅与间期细胞核和中期染色体中的骨架附着 DNA 相关。通过巯基乙醇-肌氨酸基处理，可以从复合物中解离出两个（~ 52 和~ 60）。骨架附着的和释放的 DNA 片段都含有一组紧密结合的低分子量多肽，由一个主要的 pi8 带和一个或两个次要带组成。它们似乎随机分布在整个 DNA 环中。一些在 DNA 与骨架相互作用位点紧密结合的特定蛋白质可能直接负责 DNA 与骨架的附着。
Proteins tightly bound to the DNA of mouse L cells were examined both in the regions of DNA attachment to the protein skeleton and in the DNA loops. The interphase nuclear matrix and the metaphase chromosomal scaffold, containing from 2 to 15% of the original DNA, as well as the material released from the skeleton were isolated by mild nuclease treatment. At least six proteins resistant to sarcosyl-Cs2S04 treatment are associated exclusively with skeleton-attached DNA both in interphase nuclei and metaphase chromosomes. Two (~ 52 and~ 60) can be dissociated from the complex by mercaptoethanol-sarcosyl treatment. Both skeleton-attached and released DNA fractions contain a group of tightly bound low molecular weight polypeptides consisting of a major pi8 band and one or two minor bands. They seem to be randomly distributed throughout the whole DNA loop. Some of the specific proteins that are tightly associated with DNA at the site of its interaction with the skeleton may directly be responsible for DNA attachment to the skeleton.
影响因子：
3.7
作者：
KAUFMANN, SH;COFFEY, DS;SHAPER, JH
通讯作者：
SHAPER, JH