PROPERTIES OF EXCITATORY POSTSYNAPTIC CURRENTS RECORDED INVITRO FROM RAT HIPPOCAMPAL INTERNEURONS
PROPERTIES OF EXCITATORY POSTSYNAPTIC CURRENTS RECORDED INVITRO FROM RAT HIPPOCAMPAL INTERNEURONS
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DOI：
10.1113/jphysiol.1990.sp018310
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发表时间：
1990-11-01
影响因子：
5.5
通讯作者：
NICOLL, RA
中科院分区：
文献类型：
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作者：
SAH, P;HESTRIN, S;NICOLL, RA
我们研究了刺激Schaffer侧交纤维激活的兴奋性突触电流，并使用全细胞技术记录了海马切片CA1区的中间神经元。通过差示干涉对比显微镜（DIC）和路西法黄（LY）染色观察中间神经元在细胞层外的位置和形态。兴奋性突触后电流（EPSC）有一个快速的、电压不敏感的分量和一个缓慢的、负斜率电阻在-70 ~ -40 mV之间的区域。缓慢的电压依赖性成分被n -甲基-d -天冬氨酸（NMDA）受体拮抗剂dl -2-氨基-5-磷酸戊酸（APV） 50 .mu消除。M)对快速成分影响不大。相反，快速组分被非nmda受体拮抗剂6-氰-7-硝基喹啉-2,3-二酮（CNQX; 10 .mu）消除。M)，这对慢速成分没有影响。快速组分的上升时间为1 ~ 3ms，衰减时间常数为3 ~ 15ms。慢速组分的上升时间为5 ~ 11ms，衰减时间常数为50 ~ 100ms。结果表明，虽然中间神经元上的兴奋性突触与锥体细胞上的兴奋性突触形态有很大不同，但它们的电生理和药理学性质非常相似。
We studied excitatory synaptic currents activated by stimulation of Schaffer collateral-commissuaral fibres and recorded from interneurones in the CA1 region of hippocampal slices using whole-cell techniques. Interneurones were identified by their location outside cell layer and their morphology as seen with differential interference contrast (DIC) microscopy and by filling with Lucifer Yellow (LY). The excitatory postsynaptic current (EPSC) had a fast, voltage-insensitive component and a slow component which had a region of negative slope resistance between -70 and -40 mV. The slow voltage-dependent component was abolished by the N-methyl-D-aspartate (NMDA) receptor antagonist (DL-2-amino-5-phosphonovalerate (APV) 50 .mu.M) which had little effect on the fast component. Conversely, the fast component was abolished by the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 .mu.M), which had no effect on the slow component. The rise time of the fast component ranged from 1 to 3 ms and the decay time constant ranged from 3 to 15 ms. The rise time of the slow component ranged from 5 to 11 ms and the decay time constant ranged from 50 to 100 ms. It is concluded that although the morphology of the excitatory synapses onto interneurones differs considerably from those onto pyramidal cells, their electrophysiological and pharmacological properties are very similar.