A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections.
A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections.
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DOI：
10.1039/d1cc02578c
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发表时间：
2021-06
影响因子：
4.9
通讯作者：
Jiali Zhao;Zhen Tan;Liu Wang;Chunyang Lei;Z. Nie
中科院分区：
文献类型：
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作者：
Jiali Zhao;Zhen Tan;Liu Wang;Chunyang Lei;Z. Nie
在本文中，我们描述了一种通过DNA连接反应激活的CRISPR-Cas 12 a传感平台，用于灵敏地检测非核酸靶标，包括NAD+、ATP和多核苷酸激酶（PNK）。在这种设计中，由这些生物分子触发的DNA连接反应产生DNA双链体，其可以激活Cas 12 a的核酸酶活性以产生放大的荧光信号。因此，这项工作提供了一种替代策略，将CRISPR-Cas系统的适用性扩展到检测非核酸生物分子。
Herein, we describe a CRISPR-Cas12a sensing platform activated by a DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD+, ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR-Cas system into the detection of non-nucleic acid biomolecules.