Comprehensive Evaluation for Cold Tolerance in Wucai (Brassica campestris L.) by the Performance Index on an Absorption Basis (PIabs)
Comprehensive Evaluation for Cold Tolerance in Wucai (Brassica campestris L.) by the Performance Index on an Absorption Basis (PIabs)
复制标题
基于吸收性能指数（PIabs）综合评价五彩（Brassica Campestris L.）的耐冷性
DOI：
10.3390/agronomy9020061
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发表时间：
2019-02-01
期刊：
影响因子：
3.7
通讯作者：
Wang, Chenggang
中科院分区：
文献类型：
--
作者：
Dai, Yun;Yuan, Lingyun;Wang, Chenggang
五彩是一年两次的多叶蔬菜，比大多数十字花科蔬菜对寒冷更敏感。本研究通过对五彩品种表型差异的研究，筛选出在低温条件下具有较高耐冷性的品种，为其在寒冷气候下的栽培提供依据。在PIabs的基础上，从124个基因型中选择了20个五彩型(10个低PIabs和10个高PIabs)。根据净光合速率、电解质渗漏率、冷害指数、比叶面积、丙二醛含量和PIabs等几个关键植物生理性状，从20个五彩品种中筛选出W16-13和SW-3(PIabs较高)和LS-6和W15-16(PIabs较低)。为了进一步验证PIabs的可靠性，在低温度梯度条件下培养了4个基因型，测定了它们的形态指标、叶绿素含量、总抗氧化能力、荧光瞬变(OJIP瞬变)、叶细胞超微结构和基因表达。这些指标有力地证明了PIabs较高的W16-13和SW-3具有较强的抗寒性，而LS-6和W15-16对冷害较敏感。本研究表明，易于测量的表现指数PIabs可应用于五彩品种，以筛选出一个或多个具有较高耐寒性的品种。
Wucai is a biannual leafy vegetable that is more sensitive to cold than most Brassicaceae vegetables. Here, phenotypic differences in wucai were investigated to identify those genotypes with high cold tolerance at low temperature, which could allow their cultivation in cold climates. In all, on the basis of PIabs, 20 wucai genotypes (10 lower PIabs and 10 higher PIabs) were selected from a collection of 124 genotypes. Both W16-13 and SW-3 (higher PIabs) and the LS-6 and W15-16 (lower PIabs) were screened from the 20 wucai genotypes according to several key plant physiological traits: net photosynthetic rate, electrolyte leakage, chilling injury index, specific leaf area, malondialdehyde contents, and PIabs. To further verify the reliability of PIabs, four genotypes were grown under low temperature gradient conditions and their morphological indexes, chlorophyll content, total antioxidant capacity, fluorescence transients (OJIP transients), leaf cell ultrastructure, and gene expression were measured. These indicators strongly demonstrated that the W16-13 and SW-3, which had higher PIabs, possessed higher resistance to cold stress, while both LS-6 and W15-16 were sensitive to cold. As this study shows, the easily measured performance index, PIabs, could be applied to wucai genotypes to screen for one or more varieties characterized by higher cold tolerance.