A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography
A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography
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DOI：
10.1016/j.ejmp.2016.08.012
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发表时间：
2016-12-01
影响因子：
3.4
通讯作者：
Stampanoni, Marco
中科院分区：
文献类型：
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作者：
Lovric, Goran;Mokso, Rajmund;Stampanoni, Marco
动态过程的时间分辨成像，范围从生物体内研究到原位和操作条件下的材料，需要一个灵活的终端，能够控制在不同配置和高速下相互作用的复杂组件。在X02DA TOMCAT光束线上，我们最近以20 Hz的速率实现了原位层析测量。独立地，我们已经证明了在体内肺成像到微米尺度的可行性。在本文中，我们讨论了仪器仪表的最新发展和伴随的组件，以实现这两种类型的测量。作为主要的例子，我们重点讨论了微米尺度下肺活体层析显微镜在采集方案、触发和辐射剂量方面的技术要求。我们确定了超短单次投影照射结合精确触发能力作为获得高质量重建的主要先决条件，同时限制了赋予活样本的x射线剂量。该终端提供了通用的高速成像能力，因为它与各种实验设置兼容，适合于广泛的时间分辨率研究。(C) 2016年意大利医药工业协会。Elsevier Ltd.出版。版权所有。
Time-resolved imaging of dynamic processes, ranging from biological in vivo studies to materials under in situ and in operando conditions, requires a flexible endstation capable of controlling complex components that interact in different configurations and at high speeds. At the X02DA TOMCAT beamline we have recently achieved in situ tomographic measurements at a rate of 20 Hz. Independently, we have shown the feasibility of in vivo lung imaging down to the micrometer scale. In the present paper, we discuss the latest developments in view of instrumentation and the accompanying components for achieving these two types of measurements. As the prime example, we focus on the technical requirements for in vivo tomographic microscopy of the lung at the micrometer scale in terms of acquisition schemes, triggering and radiation dose. We identify ultra-short single-projection exposures combined with accurate triggering capabilities as the main prerequisites to obtain high-quality reconstructions while limiting the X-ray dose imparted on the living sample. The presented endstation offers generic high-speed imaging capabilities, as it is compatible with a variety of experimental setups and suitable for a wide range of time-resolved studies. (C) 2016 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.