Comparative Analysis of 18S and 28S rDNA Sequences of Schistosoma japonicum from Mainland China, the Philippines and Japan
Comparative Analysis of 18S and 28S rDNA Sequences of Schistosoma japonicum from Mainland China, the Philippines and Japan
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DOI：
10.3923/javaa.2011.2010.2015
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Zhao, G. H.
中科院分区：
文献类型：
--
作者：
Chen, F.;Li, J.;Zhao, G. H.
本研究测定了中国大陆3个地理株35个日本血吸虫分离物的18S和28SrDNA部分序列，对菲律宾和日本的基因组进行扩增和比较，并用UPGMA法重建系统发育关系。使用组合的18S和28S rDNA序列以及公共数据库中可获得的属于血吸虫属的其它物种的相应序列。结果表明，所有S.日本血吸虫分离株的核苷酸序列分别为745和618 bp，遗传变异较小。japonicum菌株和分离物。系统发育分析表明，18S和28S rDNA序列不能区分S.日本血吸虫分离株的亲缘关系较近，为血吸虫种间系统发育分析和种间差异鉴定提供了有效的分子标记。
In the present study, a portion of the 18S and 28S ribosomal DNA (rDNA) sequences of 35 Schistosorna japonicum isolates representing three geographical strains from mainland China, the Philippines and Japan were amplified and compared and phylogenetic relationships were also reconstructed by Unweighted Pair-Group Method with Arithmetic averages (UPGMA) using combined 18S and 28S rDNA sequences as well as the corresponding sequences of other species belonging to the Schistosoma genus available in the public database. The results indicated that the partial 18S and 28S rDNA sequences of all S. japonicum isolates were 745 and 618 bp, respectively and displayed low genetic variation among S. japonicum strains and isolates. Phylogenetic analysis revealed that the combined 18S and 28S rDNA sequences were not able to distinguish S. japonicum isolates from three geographical origins but provided an effective molecular marker for the inter-species phylogenetic analysis and differential identification of different Schistosoma species.