Emulsan quantitation by Nile red quenching fluorescence assay
Emulsan quantitation by Nile red quenching fluorescence assay
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DOI：
10.1007/s00253-004-1849-2
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发表时间：
2005-06-01
影响因子：
5
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Castro, GR;Larson, BK;Kaplan, DL
尼罗红荧光技术定量20 - 200 μ g ml（-1）的emulsan开发。尼罗红在DMSO中的吸收峰位于552 nm，发射峰位于636 nm，摩尔消光系数为19，600 cm（-1）M-1。在DMSO溶液中，尼罗红的荧光被乳化剂按比例猝灭，猝灭具有时间依赖性。该测定法用于跟踪由威尼斯不动杆菌RAG-1的培养物产生的乳化剂。
A Nile red fluorescent technique to quantify 20 200 mu g ml(-1) of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm(-1) M-1. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.