Water stable metal-organic framework packed microcolumn for online sorptive extraction and direct analysis of naproxen and its metabolite from urine sample
Water stable metal-organic framework packed microcolumn for online sorptive extraction and direct analysis of naproxen and its metabolite from urine sample
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水稳定性金属有机框架填充微柱用于尿样中萘普生及其代谢物在线吸附萃取和直接分析
DOI：
10.1016/j.chroma.2013.04.034
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发表时间：
2013-06-14
影响因子：
4.1
通讯作者：
Li, Gongke
中科院分区：
文献类型：
--
作者：
Hu, Yuling;Song, Chaoyong;Li, Gongke
将金属-有机骨架材料MIL-101组装在聚醚醚酮（PEEK）管中作为微捕获装置，应用于尿液样品中萘普生及其代谢物的吸附萃取。MIL-101显著的水稳定性使该材料不同于其他对水分敏感的金属有机骨架。因此，它适合从生物液体中提取药物。水溶液中的吸附等温线表明，MIL-101对萘普生的吸附是吸热的。此外，MIL-101表现出比C-18键合的二氧化硅和多壁纳米管更高的对萘普生的萃取能力。特殊设计的管内吸附萃取（ITSE）装置使萃取过程具有快速、方便、易于与高效液相色谱（HPLC）联用的特点。最后将基于MIL-101的ITSE方法与HPLC和荧光检测联用，用于尿样中的萘普生和6-O-去甲基萘普生的分析。考察了样品溶液pH值、萃取流速、样品体积、解吸溶剂和时间等因素对在线萃取的影响。该方法被证明是高度敏感的线性范围为0.05-6.0 μ g L-1和检测限分别为0.034和0.011 μ g L-1的6-O-去甲基萘普生，分别。尿样中的回收率分别为853-98.3%和94.0-97.3%，日内和日间RSD分别为2.7-5.2%和7.1- 8.1%。尿液样品可以直接进行分析，无需任何额外的样品预处理。所提出的方法被证明是一种高效，灵活和通用的提取工具，非常适合与色谱方法在线结合。(C)2013爱思唯尔有限公司版权所有。
The metal-organic framework MIL-101 was fabricated in a polyetheretherketone (PEEK) tube as micro-trapping device, and applied to sorptive extraction of naproxen and its metabolite in urine samples. The remarkable water stability of the MIL-101 characterizes the material as being different from other moisture sensitive metal-organic framework. It is therefore suitable for extraction of pharmaceuticals from biological fluids. The adsorption isotherms in aqueous solution showed that the adsorption of naproxen on MIL-101 is endothermic. Additionally, MIL-101 exhibited higher extraction capacity to naproxen than that of C-18-bonded silica and multi-walled nanotube. A specially designed in-tube sorptive extraction (ITSE) device endows the extraction process with the characteristic of rapidness, convenience, and easy of conjunction with high performance liquid chromatography (HPLC). Finally the MIL-101 based ITSE method coupled with HPLC and fluorescence detection was applied to analysis of naproxen and 6-O-desmethylnaproxen in urine samples. Parameters that influence the online extraction procedure, including pH of the sample solution, flow rate of extraction, sample volume, desorption solvents and time were investigated. The method is proved to be highly sensitive with the linear range of 0.05-6.0 mu g L-1 and the limits of detection of 0.034 and 0.011 mu g L-1 for naproxen and 6-O-desmethylnaproxen, respectively. The recoveries in urine samples were 853-98.3% for naproxen and 94.0-97.3% for 6-O-desmethylnaproxen with intra- and inter-day RSDs of 2.7-5.2% and 7.1-8.1%, respectively. Urine samples could be directly subjected to analysis without any additional sample pretreatment. The proposed method was demonstrated an efficient, flexible and versatile extraction tool which is ideally suitable for online conjunction with chromatographic methods. (C) 2013 Elsevier B.V. All rights reserved.