Topography of nucleic acid helices in solutions. Steric requirements for intercalation
Topography of nucleic acid helices in solutions. Steric requirements for intercalation
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溶液中核酸螺旋的形貌。
DOI：
10.1021/ja00731a065
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
A. Depaolis
中科院分区：
文献类型：
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作者：
E. Gabbay;A. Depaolis
H, R2= CHS分别表现出强烈的低色效应（47%和41%），并且在与鲑鱼精子DNA结合时，4-硝基苯胺转变产生了较大的诱导圆二色性（b[9] ' = -6590和10,060）（表1）。(2)在32处的DNA复合体中，观察到1和2的a-甲基和b-甲基质子的pmr信号明显变宽。在DNA-2配合物的情况下，c-甲基质子的pmr信号在32和58处完全变宽，与基线噪声难以区分，表明4-硝基苯胺环2的翻滚速率有限。在90度时，DNA螺旋发生融化，在a-、b-和c-甲基质子中观察到自由报告分子特征的5条尖锐共振线。
H, R2= CHS, respectively, show a strong hypochromic effect (47 and 41%), and a large induced circular dichroism ([9]‘=—6590 and—10,060) of the 4-nitroaniline transition on binding to salmon sperm DNA (Table I).(2) Considerable broadening of the pmr signal of the a-and b-methyl protons of 1 and 2 are observed in the DNA complex at 32. 4 In the case of DNA-2 complex the pmr signal of the c-methyl protons is completely broadened and indistinguishable from base-line noise at 32 and 58, indicating a restricted tumbling rate of the 4-nitroaniline ring of 2. At 90, where melting of the DNA helix has occurred, 5 sharp resonance lines characteristic of the free reporter mole-cules are observed for the a-, b-, and c-methyl protons.