Defective tumoricidal capacity of macrophages from A/J mice. I. Characterization of the macrophage cytotoxic defect after in vivo and in vitro activation stimuli.
Defective tumoricidal capacity of macrophages from A/J mice. I. Characterization of the macrophage cytotoxic defect after in vivo and in vitro activation stimuli.
复制标题
A/J 小鼠巨噬细胞的杀肿瘤能力有缺陷。
作者：
D. Boraschi;M. Meltzer
在活化C3 H/HeN小鼠细胞的几种体内或体外治疗中的任何一种后，来自A/J小鼠的巨噬细胞不能产生杀肿瘤活性。用活的牛分枝杆菌、BCG菌株、灭活的短小棒状杆菌或吡喃共聚物腹膜内处理的A/J小鼠的腹膜巨噬细胞未能产生体外杀肿瘤活性;改变加入靶细胞的处理小鼠的巨噬细胞数量、处理剂量和时间或这些体内活化刺激物的处理时间表均未引起细胞毒性活性。此外，A/J小鼠的巨噬细胞的细胞毒性活性没有观察到任何四个靶细胞系来自三个不同的小鼠品系。用富含淋巴因子的上清液、细菌内毒素或T细胞有丝分裂原对A/J小鼠腹腔渗出液巨噬细胞进行体外治疗也是无效的;改变加入靶细胞的经治疗巨噬细胞的数量、体外激活刺激物的剂量或治疗时间均未引起细胞毒性活性。因此，A/J小鼠在体内和体外活化刺激的巨噬细胞杀肿瘤能力在广泛的实验条件下表现出深刻的缺陷。
Macrophages from A/J mice fail to develop tumoricidal activity after any of several in vivo or in vitro treatments that activate cells from C3H/HeN mice. Peritoneal macrophages from A/J mice treated i.p. with viable Mycobacterium bovis, strain BCG, killed Corynebacterium parvum, or pyran copolymer fail to develop in vitro tumoricidal activity; varying the numbers of macrophages from treated mice added to target cells, or the dose and time of treatment, or the treatment schedule of these in vivo activation stimuli did not evoke cytotoxic activity. Moreover, cytotoxic activity by macrophages from A/J mice was not observed with any of four target cell lines derived from three different mouse strains. In vitro treatment of peritoneal exudate macrophages from A/J mice with lymphokine-rich supernatants, bacterial endotoxins, or T cell mitogens was also ineffective; varying the numbers of treated macrophages added to target cells, the dose of in vitro activation stimuli, or the time of treatment did not evoke cytotoxic activity. Thus, A/J mice exhibit a profound defect in macrophage tumoricidal capacity to both in vivo and in vitro activation stimuli over a wide range of experimental conditions.