Macrophage-derived growth factor for osteoblast-like cells and chondrocytes.
Macrophage-derived growth factor for osteoblast-like cells and chondrocytes.
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用于成骨细胞样细胞和软骨细胞的巨噬细胞衍生生长因子。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
W. Peck
中科院分区：
文献类型：
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作者：
L. Rifas;V. Shen;K. Mitchell;W. Peck
原代培养的大鼠腹腔巨噬细胞对大鼠成骨样细胞和软骨细胞具有促有丝分裂因子，但对皮肤成纤维细胞没有。在巨噬细胞培养的前20小时内，在孵育培养基中出现生长促进活性，并以每种培养物中巨噬细胞数量的两倍的量释放。增殖反应后，通过DNA合成和细胞数量的增加来判断，成骨细胞样细胞富含碱性磷酸酶，这是成骨细胞特化的一个指标。巨噬细胞衍生的活性是不可透析的和热稳定的，并且通过暴露于胰蛋白酶而消除。抑制前列腺素环氧合酶未能改变其产生。部分纯化（Amicon过滤器浓缩，凝胶过滤）揭示了对应于43，000和10，000的Mr的活性主峰。根据刺激小鼠胸腺淋巴细胞增殖的能力判断，粗条件培养基和Mr 43，000峰（而非低分子量峰）表现出白细胞介素1活性。本文所述的巨噬细胞衍生生长因子可参与骨重塑和修复以及初级骨和软骨生长。
Rat resident peritoneal macrophages in primary culture were found to elaborate a mitogenic factor (or factors) for rat osteoblast-like cells and chondrocytes but not for skin fibroblasts. Growth-promoting activity appeared in the incubation medium within the first 20 hr of macrophage culture and was released in amounts that paralleled the number of macrophages per culture. After their proliferative response, as judged by increases in DNA synthesis and cell number, the osteoblast-like cells became enriched in alkaline phosphatase, an index of osteoblast specialization. The macrophage-derived activity was nondialyzable and heat-stable, and it was eliminated by exposure to trypsin. Inhibition of prostaglandin cyclooxygenase failed to modify its generation. Partial purification (Amicon filter concentration, gel filtration) disclosed principal peaks of activity corresponding to Mr of 43,000 and 10,000. The crude conditioned medium and the Mr 43,000-peak, but not the low-molecular-weight peak, exhibited interleukin 1 activity, as judged by the ability to stimulate the proliferation of mouse thymic lymphocytes. The macrophage-derived growth factor described herein may participate in bone remodeling and repair and in primary bone and cartilage growth.