Glycated hemoglobin (HbA1c) affinity biosensors with ring-shaped interdigital electrodes on impedance measurement
Glycated hemoglobin (HbA1c) affinity biosensors with ring-shaped interdigital electrodes on impedance measurement
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DOI：
10.1016/j.bios.2013.05.059
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发表时间：
2013-11-15
影响因子：
12.6
通讯作者：
Wang, Min-Haw
中科院分区：
文献类型：
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作者：
Hsieh, Ku-Meng;Lan, Kung-Chieh;Wang, Min-Haw
糖化血红蛋白（HbA₁c）是糖尿病血糖控制长期指标最重要的诊断检测方法之一。本研究提出了一种基于阻抗测量的无标记HbA₁c亲和生物传感器，它具有成本低、样本量小且实验中无需额外试剂的特点。环形叉指电极（RSIDEs）旨在提高HbA₁c的分布均匀性和固定效率，并进一步用于表征阻抗变化以及识别不同浓度的HbA₁c。提供噻吩 - 3 - 硼酸（T3BA）的自组装单分子层（SAM）来修饰金电极表面。之后，HbA₁c与T3BA之间的酯化反应在电极表面产生电性能的相对变化。带有T3BA自组装单分子层的环形叉指电极在100到10纳克/微升的宽范围内，阻抗近似呈对数下降，对HbA₁c测定的检测限低至1纳克/微升，具有良好的选择性和短期稳定性。这些显著优势（小型化和低成本）符合便携式传感器开发的即时诊断需求。版权所有©2013爱思唯尔公司。保留所有权利。
Glycated hemoglobin (HbA(1c)) is one of the most important diagnostic assays for the long-term mark of glycaemic control in diabetes. This study presents an affinity biosensor for HbA(1c) detection which is label-free based on the impedance measurement, and it features low cost, low sample volume, and requires no additional reagent in experiments. The ring-shaped interdigital electrodes (RSIDEs) are designed to promote the distribution uniformity and immobilization efficiency of HbA(1c), and are further employed to characterize the impedance change and identify various concentrations of HbA(1c). The self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is provided to modify the gold electrode surface. Afterwards, the esterification reaction between HbA(1c), and T3BA produces a relative change of electrical property on the electrode surface. The RSIDEs with SAM of T3BA exhibit a wide range from 100 to 10 ng/mu L., producing an approximate logarithmic decrease of impedance, a low detection limit of 1 ng/mu L, a good selectivity and short-term stability for HbA(1c) determination. The remarkable advantages (miniaturization and low-cost) fill the bill of point-care diagnostics for portable sensor development. (C) 2013 Elsevier B.V. All rights reserved.