Identification of a truncated cystatin SA-I as a saliva biomarker for oral squamous cell carcinoma using the SELDI ProteinChip platform
Identification of a truncated cystatin SA-I as a saliva biomarker for oral squamous cell carcinoma using the SELDI ProteinChip platform
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DOI：
10.1016/j.ijom.2009.10.001
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Toyoshima, T.
中科院分区：
文献类型：
--
作者：
Shintani, S.;Hamakawa, H.;Toyoshima, T.
需要新的、更一致的口腔鳞状细胞癌 (OSCC) 生物标志物来改善疾病的早期检测并监测患者管理。本研究的目的是检测唾液中新的 OSCC 肿瘤标志物。分析中使用了从原发性 I 期 OSCC 患者身上收集的未刺激唾液作为匹配的治疗前和治疗后样本。使用表面增强激光解吸/电离飞行时间质谱 (SELDI-TOF) ProteinChip 系统筛选唾液样本中差异表达的蛋白质。该分析揭示了 26 种蛋白质在处理前和处理后样品中表达水平显着不同 (P < 0.05)。在 OSCC 患者治疗前唾液中检测到的 14 kDa 蛋白质被鉴定为截短的半胱氨酸蛋白酶抑制剂 SA-I，其 N 末端删除了 3 个氨基酸。作者提出，蛋白质芯片分析可能为 OSCC 的早期诊断提供可靠的筛查测试，并且截短的胱抑素 SA-I 可能是 OSCC 的有用肿瘤生物标志物。
New and more consistent biomarkers of oral squamous cell carcinoma (OSCC) are needed to improve early detection of the disease and to monitor patient management. The aim of this Study was to detect new OSCC tumor markers in saliva. Unstimulated saliva, collected from patients with primary stage I OSCC as matched pre-and post-treatment samples, was used in the analysis. A surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF) ProteinChip system was used to screen for differentially expressed proteins in the saliva samples. This analysis revealed 26 proteins with significantly different expression levels in the pre-and post-treatment samples (P < 0.05). A 14 kDa protein detected in pre-treatment saliva from the OSCC patients was identified as a truncated cystatin SA-I, with deletion of three amino acids from the N-terminus. The authors propose that ProteinChip analysis may provide a reliable screening test for early diagnosis of OSCC and that truncated cystatin SA-I might be a useful tumor biomarker for OSCC.