RELATION BETWEEN ANTIBODY-RESPONSE TO PSEUDOMONAS-AERUGINOSA EXOPROTEINS AND COLONIZATION INFECTION IN PATIENTS WITH CYSTIC-FIBROSIS
RELATION BETWEEN ANTIBODY-RESPONSE TO PSEUDOMONAS-AERUGINOSA EXOPROTEINS AND COLONIZATION INFECTION IN PATIENTS WITH CYSTIC-FIBROSIS
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DOI：
10.1111/j.1651-2227.1984.tb17774.x
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
VASIL, ML
中科院分区：
文献类型：
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作者：
GRANSTROM, M;ERICSSON, A;VASIL, ML
采用酶联免疫吸附试验(EL ISA)检测囊性纤维化患者对铜绿假单胞菌外毒素A、弹性蛋白酶、碱性蛋白酶和磷脂酶C的抗体反应。仅慢性定植患者的磷脂酶C(22/22例)、碱性蛋白酶(16/22例)、外毒素A(15/22例)和弹力酶(5/22例)的抗体纤维升高。在少数有连续标本的患者中，在活动性感染期间，所有4种抗原的滴度都有上升的记录。经抗生素治疗后，4种抗原的滴度均下降，但仅抗外毒素A和抗弹性蛋白酶滴度降至正常水平。抗生素治疗对磷脂酶C效价的影响最小。这些结果暗示了这些外源蛋白在慢性定植和活动性感染中的不同作用。铜绿假单胞菌对外源蛋白的抗体水平可用于监测CF患者的治疗情况。
Enzyme-linked immunosorbent assay (ELISA) was used to measure the antibody response to P. aeruginosa exotoxin A, elastase, alkaline protease and phospholipase C in patients with cystic fibrosis (CF). Only the chronically colonized patients showed elevated antibody fibers to phospholipase C (22/22 patients), alkaline protease (16/22 patients), exotoxin A (15/22 patients) and elastase (5/22 patients). In a few patients where serial specimens were available, rising titers were recorded to all 4 antigens during periods of active infection. Antibiotic treatment resulted in decrease of titers against all 4 antigens, but only the anti-exotoxin A and anti-elastase titers decreased to normal levels. Titers to phospholipase C were the least influenced by antibiotic treatment. The results imply different roles for these exoproteins in chronic colonization vs. active infection. The levels of P. aeruginosa antibodies to exoproteins could probably be used in monitoring treatment of patients with CF.