AtDPG1 is involved in the salt stress response of Arabidopsis seedling through ABI4
AtDPG1 is involved in the salt stress response of Arabidopsis seedling through ABI4
复制标题
AtDPG1通过ABI4参与拟南芥幼苗的盐胁迫反应
DOI：
10.1016/j.plantsci.2019.110180
复制
发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Liu Dong
中科院分区：
文献类型：
--
作者：
Yi Jian;Zhao Dongming;Chu Jinfang;Yan Jijun;Liu Jinsong;Wu Meijia;Cheng Jianfeng;Jiang Haiyan;Zeng Yongjun;Liu Dong
虽然控制叶绿体发育的基因在植物对环境胁迫的反应中起着重要作用，但其分子机制仍不清楚。在这项研究中，一个拟南芥突变dpg 1（延迟pale-greening 1）与叶绿体发育缺陷进行了研究。通过定量RT-PCR和GUS组织化学分析，证明AtDPG 1主要在拟南芥幼苗的绿色组织中表达，并能受到盐胁迫的诱导。表型分析表明，AtDPG 1基因突变增强了拟南芥幼苗对盐胁迫的敏感性。进一步的研究表明，拟南芥AtDPG 1基因的缺失以ABA依赖的方式增加了拟南芥对盐胁迫的敏感性。此外，NaCl处理显著改变了dpg 1植株中各种胁迫响应基因和阿坝信号相关基因的表达水平。值得注意的是，ABI 4 indpg 1突变体在盐条件下的转录水平比野生型植物显著增加。dpg 1/abi 4双突变体幼苗在盐胁迫下表现出比dpg 1单突变体更强的抗盐性，表明dpg 1幼苗的盐敏感表型可以通过ABI 4功能的丧失而得到拯救。这些结果表明AtDPG 1通过ABI 4参与了拟南芥幼苗的盐胁迫响应。
Although the genes controlling chloroplast development play important roles in plant responses to environmental stresses, the molecular mechanisms remain largely unclear. In this study, an Arabidopsis mutantdpg1(delayed pale-greening1) with a chloroplast development defect was studied. By using quantitative RT-PCR and histochemical GUS assays, we demonstrated thatAtDPG1was mainly expressed in the green tissues of Arabidopsis seedlings and could be induced by salt stress. Phenotypic analysis showed that mutation inAtDPG1lead to an enhanced sensitivity to salt stress in Arabidopsis seedlings. Further studies demonstrated that disruption of theAtDPG1in Arabidopsis increases its sensitivity to salt stress in an ABA-dependent manner. Moreover, expression levels of various stress-responsive and ABA signal-related genes were remarkably altered in thedpg1plants under NaCl treatment. Notably, the transcript levels ofABI4indpg1mutant increased more significantly than that in wild type plants under salt conditions. The seedlings ofdpg1/abi4double mutant exhibited stronger resistance to salt stress after salt treatment compared with thedpg1single mutant, suggesting that the salt-hypersensitive phenotype ofdpg1seedlings could be rescued via loss of ABI4 function. These results reveal that AtDPG1 is involved in the salt stress response of Arabidopsis seedling through ABI4.