STRUCTURE AND EXPRESSION OF THE HUMAN L-MYC GENE REVEAL A COMPLEX PATTERN OF ALTERNATIVE MESSENGER-RNA PROCESSING
STRUCTURE AND EXPRESSION OF THE HUMAN L-MYC GENE REVEAL A COMPLEX PATTERN OF ALTERNATIVE MESSENGER-RNA PROCESSING
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DOI：
10.1128/mcb.8.1.186
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发表时间：
1988-01-01
影响因子：
5.3
通讯作者：
MINNA, J
中科院分区：
文献类型：
--
作者：
KAYE, F;BATTEY, J;MINNA, J
我们详细分析了从人胎盘DNA中分离到的L-myc基因的结构，并鉴定了其在几种小细胞肺癌细胞系中的表达。该基因由三个外显子和两个内含子组成，在人类DNA中跨度为6.6kb。在所有表达L-myc的小细胞肺癌细胞系中都产生了几种不同的mRNAs。这些转录本是通过内含子1和内含子2的交替剪接以及通过交替使用多聚腺苷酸化信号从单个基因产生的。在一些mRNA中，有一个长的开放阅读框架，预测的翻译蛋白长达364个残基。与c-myc和N-myc的氨基酸序列比较显示有多个离散区域，具有广泛的同源性。相比之下，通过替代加工产生的其他mRNA转录本可以编码带有新的羧基末端的截断蛋白质。
We analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs ther is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.