Possible involvement of oxidative stress in salivary gland of patients with Sjogren's syndrome
Possible involvement of oxidative stress in salivary gland of patients with Sjogren's syndrome
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DOI：
10.1159/000098211
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发表时间：
2006-01-01
期刊：
影响因子：
5
通讯作者：
Saito, Ichiro
中科院分区：
文献类型：
--
作者：
Ryo, Koufuchi;Yamada, Hiroyuki;Saito, Ichiro
目的：确定干燥综合征（SS）患者唾液腺氧化应激的参与情况。方法：使用 SS 唾液通过 8-羟基-2'-脱氧鸟苷 (8-OHdG) 和己酰赖氨酸 (HEL) 测量腺体的氧化损伤。此外，还分析了乳酸脱氢酶（LDH）和线粒体谷氨酸草酰乙酸转氨酶（m-GOT）这两种细胞损伤的一般标志物。结果：SS 患者唾液中 8-OHdG 和 HEL 水平升高，但其他唾液腺功能障碍患者或健康个体的唾液中 8-OHdG 和 HEL 水平没有升高。 LDH 和 m-GOT 水平分别与 8-OHdG 和 HEL 水平显着相关。此外，SS 唾液中 8-OHdG 和 HEL 水平的增加也相关。结论：这些发现表明氧化应激参与了 SS 的腺体组织破坏。提示唾液中8-OHdG和HEL的检测可能成为SS诊断的有用工具。版权所有 (c) 2006 S. Karger AG，巴塞尔。
Objective: To determine the involvement of oxidative stress in the salivary gland of patients with Sjogren's syndrome ( SS). Methods: Oxidative damage to the gland was measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) and hexanoyllysine (HEL) using the SS saliva. In addition, lactate dehydrogenase (LDH) and mitochondrial glutamic-oxaloacetic transaminase (m-GOT), both general markers for cell damage, were also analyzed. Results: Increased levels of 8-OHdG and HEL were found in the saliva of SS patients, but not in that of patients with other salivary gland dysfunction or of healthy individuals. Levels of LDH and m-GOT were significantly correlated with 8-OHdG and HEL levels, respectively. Furthermore, the increased levels of 8-OHdG and HEL were also correlated in the SS saliva. Conclusion: These findings suggested the involvement of oxidative stress in glandular tissue destruction in SS. It was indicated that the detection of 8-OHdG and HEL in the saliva may become a useful tool for the diagnosis of SS. Copyright (c) 2006 S. Karger AG, Basel.