Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea
Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea
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DOI：
10.3168/jds.s0022-0302(07)71604-1
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Koo, H. C.
中科院分区：
文献类型：
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作者：
Moon, J. -S.;Lee, A. -R.;Koo, H. C.
金黄色葡萄球菌属于主要的传染性乳腺炎病原体，而凝固酶阴性葡萄球菌（CNS）也能引起机会性牛乳腺炎。由于在农业和医疗保健环境中长期使用 β-内酰胺抗生素，其中许多菌株对青霉素或氨苄西林具有耐药性。基于 PCR-RFLP 的简单且高度特异性的凝固酶基因分型，用于区分葡萄球菌。本研究确定了金黄色葡萄球菌表型抗菌谱与凝固酶基因多态性之间的关系。 1997 年至 2004 年间，从韩国 8 个省 153 个奶牛场的 3,047 个牛乳腺奶样品中分离出葡萄球菌菌株（835 个金黄色葡萄球菌和 763 个 CNS）。二十一 (2.5%) 葡萄球菌。金黄色葡萄球菌和19株（2.4%）CNS菌株对甲氧西林耐药[苯唑西林最低抑菌浓度（MIC）≥4μg/mL]。 mecA 基因也在 13 种耐甲氧西林葡萄球菌中发现。金黄色葡萄球菌 (MRSA) 和 12 种耐甲氧西林中枢神经系统 (MRCNS) 分离株，与 MIC < 8 μg/mL 的 MRSA 相比，高 MIC (>= 16 μg/mL) 的 MRSA 中 mecA 基因的检出率显着更高。耐甲氧西林葡萄球菌。金黄色葡萄球菌和 MRCNS 对其他抗生素（氨苄青霉素、头孢菌素、卡那霉素和庆大霉素）的耐药性也比对甲氧西林敏感的葡萄球菌更强。 706 葡萄球菌中有 10 种不同的 coa PCR-RFLP 模式（A 到 J）。金黄色葡萄球菌菌株中，主要类型为A型（26.9％）、B型（17.0％）、G型（10.5％）和H型（15.4％），其中A型和H型（6和10株）在MRSA中常见。这项研究表明主要流行葡萄球菌。金黄色葡萄球菌克隆可能在不同奶牛场之间传播，coa基因型谱可用于牛乳腺炎的流行病学调查和控制，特别是由具有特定流行coa类型的MRSA引起的牛乳腺炎。
Staphylococcus aureus belongs to the group of major contagious mastitis pathogens, whereas the coagulase-negative staphylococci (CNS) are also capable of causing opportunistic bovine mastitis. Many of these strains are resistant to penicillin or ampicillin because of the long-term use of beta-lactam antibiotics in agricultural and healthcare settings. Based on the simple and highly specific coagulase genotyping by PCR-RFLP used for discriminating among Staph. aureus strains, the relationship between phenotypic antibiogram and the polymorphism of coagulase gene was determined in this study. The staphylococci strains ( 835 Staph. aureus and 763 CNS) were isolated from 3,047 bovine mastitic milk samples from 153 dairy farms in 8 provinces from 1997 to 2004 in the Republic of Korea. Twenty-one (2.5%) Staph. aureus and 19 (2.4%) CNS strains were resistant to methicillin [ oxacillin minimum inhibitory concentration (MIC) >= 4 mu g/mL]. The mecA gene was also found in 13 methicillin-resistant Staph. aureus (MRSA) and 12 methicillin-resistant CNS (MRCNS) isolates with a significantly higher detection rate of the mecA gene in MRSA with high MIC (>= 16 mu g/mL) compared with those with MIC < 8 mu g/mL. Methicillin-resistant Staph. aureus and MRCNS were also more resistant to other antibiotics ( ampicillin, cephalothin, kanamycin, and gentamicin) than methicillin-susceptible staphylococci. Among 10 different coa PCR-RFLP patterns ( A to J) in 706 Staph. aureus strains, the main types were A (26.9%), B (17.0%), G (10.5%), and H (15.4%), with the frequent observation of the A and H types ( 6 and 10 isolates) in MRSA. This study indicates that major epidemic Staph. aureus clones may be spread between different dairy farms, and the profile of coa genotype can be applied for epidemiological investigations and control of bovine mastitis, particularly one caused by MRSA with specific prevalent coa types.