Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method
Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method
复制标题
DOI：
10.1590/s0100-40422013000700020
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Kara, Huseyin
中科院分区：
文献类型：
--
作者：
Kucukkolbasi, Semahat;Bilber, Onur;Kara, Huseyin
在本研究中，反相高效液相色谱法（RP-HPLC）的程序开发和验证的同时测定7个水溶性维生素（硫胺素，核黄素，烟酸，氰钴胺，抗坏血酸，叶酸，和对氨基苯甲酸）和4个脂溶性维生素（视黄醇醋酸酯，胆钙化醇，α-生育酚，和植物甲萘醌）的复合维生素片。在10-500 ng/mL范围内，线性关系良好（R-2 > 0.999）。通过进行日内和日间精密度，对该方法进行统计学评价。通过测量平均回收率检测方法的准确度;值范围为87.4%-98.5%，是符合标示量的可接受定量结果。
In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, alpha-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R-2 > 0.999) over the concentration range of 10-500 ng mL(-1). The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.