The effect of temperature on phenolic content in wounded carrots
The effect of temperature on phenolic content in wounded carrots
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温度对受伤胡萝卜酚类物质含量的影响
DOI：
10.1016/j.foodchem.2016.07.172
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发表时间：
2017-01-15
期刊：
影响因子：
8.8
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Han, Cong;Li, Jing;Zheng, Yonghua
活性氧（ROS）在损伤胡萝卜酚类抗氧化剂的生物合成中起重要作用。本研究进一步了解了贮藏温度对伤胡萝卜中酚类物质积累的影响。结果表明，损伤强度的增加和贮藏温度的升高均促进了损伤胡萝卜体内活性氧的生成和酚类物质的积累。此外，ROS抑制剂抑制了ROS的生成，抑制了苯丙氨酸途径关键酶（苯丙氨酸解氨酶，PAL，肉桂酸-4-羟化酶，C4H， 4-香豆酸辅酶A连接酶，4CL）的活性，抑制了胡萝卜丝中酚类物质的积累，证实了之前的报道。与此相反，ROS激发剂处理促进了ROS的产生，增强了PAL、C4H和4CL的活性，并诱导了酚类物质的积累。因此，我们的研究结果证实了活性氧在介导胡萝卜损伤诱导的酚类物质积累中是必不可少的，并表明温度升高通过诱导活性氧的产生促进了酚类物质的积累。(C) 2016 Elsevier Ltd.版权所有。
Reactive oxygen species (ROS) have been shown to play important roles in biosynthesis of phenolic antioxidants in wounded carrots. This study has gone further to understand the effects of storage temperature on phenolics accumulation in wounded carrots. The results indicated that both increased wounding intensity and higher storage temperature promoted the generation of ROS and enhanced phenolics accumulation in wounded carrots. Moreover, treatment with ROS inhibitor inhibited ROS generation, suppressed the activities of key enzymes in phenylpropanoid pathway (phenylalanine ammonia lyase, PAL; cinnamate-4-hydroxylase, C4H; 4-coumarate coenzyme A ligase, 4CL) and restrained phenolics accumulation in shredded carrots confirming previous reports. In contrast, treatment with ROS elicitor promoted ROS generation, enhanced the activities of PAL, C4H and 4CL, and induced phenolics accumulation. Thus, our results confirmed that ROS are essential for mediating wound-induced phenolics accumulation in carrots and suggested that increase temperature enhanced the accumulation of phenolics through inducing ROS generation. (C) 2016 Elsevier Ltd. All rights reserved.