INTRANUCLEAR FILAMENTS CONTAINING A NUCLEAR-PORE COMPLEX PROTEIN
INTRANUCLEAR FILAMENTS CONTAINING A NUCLEAR-PORE COMPLEX PROTEIN
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DOI：
10.1083/jcb.123.6.1333
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发表时间：
1993-12-01
影响因子：
7.8
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
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作者：
CORDES, VC;REIDENBACH, S;FRANKE, WW
核孔复合物（Nuclear pore complex，NPC）是直径为3-6 nm的核内细丝的锚定位点，这些细丝同轴地排列在圆柱体的周边上，并且在不同种类的细胞中以不同的长度突出到核内部。使用特异性单克隆抗体，我们发现在SDS-PAGE上大约190 kD的多肽，其似乎与最近描述的NPC蛋白“nup 153”相同，是来自不同物种的不同类型细胞中这些核内NPC附着丝的一般成分，包括两栖类卵母细胞，其中这些丝丰富并且可以相对较长。我们进一步观察到，在有丝分裂过程中，这种丝蛋白瞬时解体，导致一个独特的可溶性分子实体约12.5 S，然后分散在大部分的细胞质。同样，两栖类卵母细胞蛋白出现在一个可溶性的形式约16 S在减数分裂中期，可以从卵细胞质上清液免疫沉淀。我们的结论是，这种NPC蛋白可以组装成一个丝状的形式在相当大的距离核膜和讨论可能的功能，这些NPC附着丝，从一个角色作为指导结构参与核质运输的一种形式的过量存储的NPC蛋白在卵母细胞。
Nuclear pore complexes (NPCs) are anchoring sites of intranuclear filaments of 3-6 nm diameter that are coaxially arranged on the perimeter of a cylinder and project into the nuclear interior for lengths varying in different kinds of cells. Using a specific monoclonal antibody we have found that a polypeptide of approximately 190 kD on SDS-PAGE, which appears to be identical to the recently described NPC protein ''nup 153,'' is a general constituent of these intranuclear NPC-attached filaments in different types of cells from diverse species, including amphibian oocytes where these filaments are abundant and can be relatively long. We have further observed that during mitosis this filament protein transiently disassembles, resulting in a distinct soluble molecular entity of approximately 12.5 S, and then disperses over most of the cytoplasm. Similarly, the amphibian oocyte protein appears in a soluble form of approximately 16 S during meiotic metaphase and can be immunoprecipitated from egg cytoplasmic supernatants. We conclude that this NPC protein can assemble into a filamentous form at considerable distance from the nuclear envelope and discuss possible functions of these NPC-attached filaments, from a role as guidance structure involved in nucleocytoplasmic transport to a form of excess storage of NPC proteins in oocytes.