Screening for polyhydroxyalkanoate (PHA)-producing bacterial strains and comparison of PHA production from various inexpensive carbon sources
Screening for polyhydroxyalkanoate (PHA)-producing bacterial strains and comparison of PHA production from various inexpensive carbon sources
复制标题
DOI：
10.1007/s13213-010-0181-6
复制
发表时间：
2011-09-01
影响因子：
3
通讯作者：
Hasnain, Shahida
中科院分区：
文献类型：
--
作者：
Chaudhry, Waqas Nasir;Jamil, Nazia;Hasnain, Shahida
共20个不同的菌株进行分离，纯化和筛选的聚羟基链烷酸酯（PHA）的生产。通过尼罗蓝染色筛选PHA产生菌，并通过苏丹黑B染色进行确认。选择菌株1.1用于进一步分析，因为其高PHA生产能力。优化PHA生产并计算时间曲线。在各种不同的廉价碳源上的PHA生产，即，制糖工业废物（发酵醪，糖蜜，废洗涤）和玉米油进行了比较。计算并比较细胞干重和PHA含量（%）。12.53g/L为该菌株在含玉米油培养基中生长时的CDW。结果表明，12.53 g/L的玉米油可以作为细菌生长的碳源，使细胞积累PHA高达35.63%。扩增PhaC基因以确认产生PHA的遗传基础。16 S rRNA基因序列分析表明菌株1.1属于假单胞菌属。
A total of 20 different strains were isolated, purified and screened for polyhydroxyalkanoate (PHA) production. PHA-producing strains were screened by Nile blue staining and confirmed by Sudan Black B staining. Strain 1.1 was selected for further analysis due to its high PHA production ability. PHA production was optimized and time profiling was calculated. PHA production on various different cheap carbon sources, i.e., sugar industry waste (fermented mash, molasses, spent wash) and corn oil, was compared. Cell dry weight and PHA content (%) were calculated and compared. The 12.53 g/L is the CDW of bacterial strain when grown in medium containing corn oil. It was found that corn oil at 12.53 g/L medium can serve as a carbon source for bacterial growth, allowing cells to accumulate PHA up to 35.63 %. The PhaC gene was amplified to confirm the genetic basis for the production of PHAs. Moreover, 16S rRNA gene sequence analysis showed that strain 1.1 belongs to Pseudomonas species.