Antioxidant activity of berry phenolics on human low-density lipoprotein and liposome oxidation
Antioxidant activity of berry phenolics on human low-density lipoprotein and liposome oxidation
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DOI：
10.1021/jf980181c
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发表时间：
1998-10-01
影响因子：
6.1
通讯作者：
Frankel, EN
中科院分区：
文献类型：
--
作者：
Heinonen, IM;Meyer, AS;Frankel, EN
浆果中存在的酚类化合物的抗氧化活性进行了研究，通过两个铜催化的体外氧化试验：人低密度脂蛋白（LDL）和卵磷脂脂质体。新鲜浆果中总酚的量在617和4350毫克/公斤之间变化，作为没食子酸当量（GAE）。在低密度脂蛋白在10 μ M的GAE，浆果提取物抑制己醛的形成的顺序：黑莓>红树莓>甜樱桃，蓝莓>草莓。在卵磷脂脂质体中，提取物抑制己醛形成的顺序为：甜樱桃>蓝莓>红树莓>黑莓>草莓。红树莓比蓝莓更有效地抑制卵磷脂脂质体中氢过氧化物的形成。高效液相色谱分析表明，高花青素含量的黑莓，羟基肉桂酸的蓝莓和甜樱桃，黄酮醇的蓝莓，和黄烷-3-醇的红树莓。对低密度脂蛋白的抗氧化活性与花色苷直接相关，与黄酮醇间接相关，对脂质体的抗氧化活性与羟基肉桂酸含量相关。因此，浆果是可能对健康有潜在影响的酚类抗氧化剂的重要来源。
The antioxidant activity of phenolic compounds present in berries was investigated by two copper-catalyzed in vitro oxidation assays: human low-density lipoproteins (LDL) and lecithin liposomes. The amount of total phenolics varied between 617 and 4350 mg/kg in fresh berries, as gallic acid equivalents (GAE). In LDL at 10 mu M GAE, berry extracts inhibited hexanal formation in the order: blackberries > red raspberries > sweet cherries, blueberries > strawberries. In lecithin liposomes, the extracts inhibited hexanal formation in the order: sweet cherries > blueberries > red raspberries > blackberries > strawberries. Red raspberries were more efficient than blueberries in inhibiting hydroperoxide formation in lecithin liposomes. HPLC analyses showed high anthocyanin content in blackberries, hydroxycinnamic acid in blueberries and sweet cherries, flavonol in blueberries, and flavan-3-ol in red raspberries. The antioxidant activity for LDL was associated directly with anthocyanins and indirectly with flavonols, and for liposome it correlated with the hydroxycinnamate content. Berries thus contribute a significant source of phenolic antioxidants that may have potential health effects.