Effect of recombinant and purified hematopoietic growth factors on human megakaryocyte colony formation.
Effect of recombinant and purified hematopoietic growth factors on human megakaryocyte colony formation.
复制标题
重组和纯化的造血生长因子对人巨核细胞集落形成的影响。
作者：
E. Bruno;Briddell Ra;R. Hoffman
多种纯化或重组造血生长因子的作用，包括重组促红细胞生成素 (rEpo)、血小板生成刺激因子 (TSF)、重组白细胞介素 1 α (rIL-1 α)、重组粒细胞集落刺激因子 (rG-CSF)、巨噬细胞集落刺激因子 (CSF-1)、重组白细胞介素 3 (rIL-3) 和重组粒细胞巨噬细胞测定了集落刺激因子（rGM-CSF）对正常人骨髓细胞在去血清测定系统中形成巨核细胞（MK）集落的影响。单独使用 rEpo、TSF、CSF-1、rIL-1 α 或 rG-CSF 均不会增强 MK 集落形成。最佳剂量的 rGM-CSF 和 rIL-3 均使 MK 集落形成比基线值分别增加八倍和十倍。添加越来越多的rGM-CSF或rIL-3会导致形成的MK集落数量逐渐增加，直到达到平台水平。 rGM-CSF 和 rIL-3 也导致培养物中形成的每个 MK 集落的细胞数量呈剂量相关增加。当比较它们在最佳浓度下的效果时，这些分子是 MK 集落形成的等效刺激剂。 rGM-CSF和rIL-3的作用在rIL-3的次优浓度下是相加的，因为两种生长因子的组合形成的集落形成近似于单独的每种生长因子形成的集落形成的总和。这些数据表明，rGM-CSF和rIL-3单独或组合是祖细胞水平的体外巨核细胞生成的重要调节剂。
The effect of a number of purified or recombinant hematopoietic growth factors, including recombinant erythropoietin (rEpo), thrombocytopoiesis stimulating factor (TSF), recombinant interleukin 1 alpha (rIL-1 alpha), recombinant granulocyte colony-stimulating factor (rG-CSF), macrophage colony-stimulating factor (CSF-1), recombinant interleukin 3 (rIL-3), and recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF), on megakaryocyte (MK) colony formation by normal human marrow cells in a serum-depleted assay system was determined. Neither rEpo, TSF, CSF-1, rIL-1 alpha, nor rG-CSF alone augmented MK colony formation. Both rGM-CSF and rIL-3 at optimal doses increased MK colony formation eightfold and tenfold, respectively, above baseline values. Addition of increasing amounts of either rGM-CSF or rIL-3 led to progressively greater numbers of MK colonies formed until plateau levels were reached. Both rGM-CSF and rIL-3 also led to a dose-related increase in the number of cells per MK colony formed in culture. These molecules were equivalent stimulators of MK colony formation when their effects at optimal concentrations were compared. The effects of rGM-CSF and rIL-3 were additive at suboptimal concentrations of rIL-3 in that colony formation by a combination of the two growth factors approximated the sum of colony formation by each growth factor alone. These data suggest that rGM-CSF and rIL-3 alone and in combination are important regulators of in vitro megakaryocytopoiesis at the progenitor cell level.