Potential and performance of accelerated solvent extraction (ASE) in obtaining bioactive compounds from bee propolis: Comparison with soaking, ultrasonication and microwave-assisted methods
Potential and performance of accelerated solvent extraction (ASE) in obtaining bioactive compounds from bee propolis: Comparison with soaking, ultrasonication and microwave-assisted methods
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加速溶剂萃取 (ASE) 从蜂胶中获取生物活性化合物的潜力和性能：与浸泡、超声波和微波辅助方法的比较
DOI：
10.11594/jtls.11.02.08
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Mojica, Elmer-Rico E.
中科院分区：
文献类型：
--
作者：
Charland, Jozlyn;Deocaris, Custer C.;Micor, J.R.L.;Mojica, Elmer-Rico E.
蜂胶是蜜蜂从树木的幼芽和分泌物中采集的一种天然分泌物。近年来，由于其具有抗菌、抗氧化、抗溃疡等多种生物学特性，作为功能性食品成分受到广泛关注。在这项研究中，加速溶剂提取（ASE）的性能进行了评估，并与不同的提取方法：浸泡（浸渍），超声波，微波辅助方法进行了比较。采用气相色谱-质谱联用（GC-MS）和吸光度、荧光等光谱技术对天然产物的提取效率进行了评价。同时测定了不同提取物的抗氧化活性和酚类物质含量。结果表明，微波法提取的样品中生物活性物质的得率最高。虽然微波是本研究中最好的方法，但对ASE应用于蜂蜂胶天然产物提取的一些问题和建议进行了讨论。由于ASE提取温度容易控制，因此，该技术具有很大的潜力，可作为一种更好的从植物中提取热不稳定天然产物的方法，但仍需进一步优化提取条件。
Propolis is a natural resinous substance collected by honeybees from buds and exu-dates of trees. The material has attracted much attention in recent years as a functional food component since it possesses various biological properties, including antimicro-bial, antioxidative, and anti-ulcer properties. In this study, the performance of accel-erated solvent extraction (ASE) was assessed and compared with varying methods of extraction: soaking (maceration), ultrasonication, and microwave-assisted methods. Gas chromatography-mass spectrometry (GC-MS) and other spectroscopic tech-niques, such as absorbance and fluorescence, were employed to assess the efficiency in the extraction of natural products. The antioxidant activity and phenolic content of the different extracts were also determined. Results showed samples obtained from the microwave method showed the highest yield in the extraction of bioactive com-pounds. Although microwave showed the best method in this study, some issues and recommendations on ASE application for extracting natural products from bee prop-olis were discussed. Given the ease in controlling extraction temperature with ASE, this technique has a great potential to be a better method for extraction of heat-labile natural products from propolis should optimization of conditions for extraction were further performed.