The New Matrix 4-Chloro-α-Cyanocinnamic Acid Allows the Detection of Phosphatidylethanolamine Chloramines by MALDI-TOF Mass Spectrometry
The New Matrix 4-Chloro-α-Cyanocinnamic Acid Allows the Detection of Phosphatidylethanolamine Chloramines by MALDI-TOF Mass Spectrometry
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DOI：
10.1016/j.jasms.2008.12.028
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发表时间：
2009-05-01
影响因子：
3.2
通讯作者：
Schiller, Juergen
中科院分区：
文献类型：
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作者：
Jaskolla, Thorsten;Fuchs, Beate;Schiller, Juergen
磷脂酰乙醇胺（PE）是细胞膜上丰富的脂质成分。PE的氨基与体内炎症条件下产生的次氯酸具有高反应性。分析所得的PE单和二氯胺是显着的利益，因为这些物种代表重要的介质脂质过氧化。我们已经表明，在以前的通信，PE氯胺的质谱检测是唯一可能的ESI MS，而MALDI-TOF MS无法检测到这些产品，如果使用标准matrixs.In这项工作中，我们证明，PE氯胺的检测也是可能的MALDI-TOF MS，如果4-chloro-alpha-cyanocinnamic acid被用作矩阵。将详细讨论导致这些物质电离的基本过程。考虑到可能的分子内重排进行了实验和理论研究，以澄清这些方面。（J Am Soc Mass Spectrom 2009，20，867-874）（C）2009由Elsevier Inc.美国质谱学会American Society for Mass Spectrometry
Phosphatidylethanolamines (PEs) are abundant lipid constituents of the cellular membrane. The amino group of PEs exhibits high reactivity with hypochlorous acid that is generated under inflammatory conditions in vivo. The analysis of the resulting PE mono- and dichloramines is of significant interest since these species represent important mediators of lipid peroxidation. We have shown in a previous communication that mass spectrometric detection of PE chloramines is only possible with ESI MS, whereas MALDI-TOF MS fails to detect these products if standard matrices are used.In this work we demonstrate that the detection of PE chloramines is also possible by MALDI-TOF MS if 4-chloro-alpha-cyanocinnamic acid is used as matrix. The underlying processes leading to ionization of these species will be discussed in detail. Both, experimental and theoretical Studies taking into account possible intramolecular rearrangements were performed to clarify these aspects. (J Am Soc Mass Spectrom 2009, 20, 867-874) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry