Enhanced endothelialization for developing artificial vascular networks with a natural vessel mimicking the luminal surface in scaffolds
Enhanced endothelialization for developing artificial vascular networks with a natural vessel mimicking the luminal surface in scaffolds
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DOI：
10.1016/j.actbio.2012.08.042
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发表时间：
2013-01-01
影响因子：
9.7
通讯作者：
Cho，Dong-Woo
中科院分区：
文献类型：
--
作者：
Kang，Tae-Yun;Hong，Jung Min;Cho，Dong-Woo
由于缺乏氧气和营养供应，大组织再生仍然存在问题。为了满足细胞的代谢需要，已经在支架内预先形成分支血管网络，作为物质运输的通道。当构建具有通道通畅的功能性血管网络时，重点应放在抗血栓表面问题上。本研究的目的是开发一种快速内皮化方法，用于在人工血管网络中模拟自然血管壁创建抗血栓形成的表面。剪切应力预处理和支架表面修饰是促进生物材料内皮化的有效途径。我们发现，在适当的时间短暂增加剪切应力是促进内皮化的关键。此外，生物活性材料如胶原蛋白和重组贻贝粘附蛋白与精氨酸-甘氨酸-天冬氨酸肽（MAP-RGD）融合的表面修饰与剪切应力预处理具有协同作用。血小板粘附试验表明MAP-RGD本身没有内皮化的抗血栓形成潜力。本研究建立的快速内皮化方法可以很容易地应用于多孔支架中预制的人工血管网络。具有抗血栓形成管腔表面的人工血管网络的开发将为组织工程和再生医学开辟新的篇章。
Large tissue regeneration remains problematic because of a lack of oxygen and nutrient supply. An attempt to meet the metabolic needs of cells has been made by preforming branched vascular networks within a scaffold to act as channels for mass transport. When constructing functional vascular networks with channel patency, emphasis should be placed on anti-thrombogenic surface issues. The aim of this study was to develop a rapid endothelialization method for creating an anti-thrombogenic surface mimicking the natural vessel wall in the artificial vascular networks. Shear stress preconditioning and scaffold surface modification were investigated as effective approaches for promoting biomaterial endothelialization. We found that a transient increase in shear stress at the appropriate time is key to enhancing endothelialization. Moreover, surface modification with bioactive materials such as collagen and recombinant mussel adhesive protein fused with arginine–glycine–aspartic acid peptide (MAP-RGD) showed a synergetic effect with shear stress preconditioning. Platelet adhesion tests demonstrated the anti-thrombogenic potential of MAP-RGD itself without endothelialization. The rapid endothelialization method established in this study can be easily applied to preformed artificial vascular networks in porous scaffolds. Development of artificial vascular networks with an anti-thrombogenic luminal surface will open up a new chapter in tissue engineering and regenerative medicine.